Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

324
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

84
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DISTRIBUTION OF INSERTION SEQUENCES ASSOCIATED WITH TN1546 AND CLONAL DIVERSITY OF VANCOMYCIN-RESISTANT ENTEROCOCCI ISOLATED FROM PATIENTS IN TEHRAN, IRAN

Pages

  15-22

Abstract

 Background and objectives: Infection with vancomycin-resistant enterococci (VRE) has caused a therapeutic problem. Van A and Van B resistant types are the predominant phenotypes among vancomycin resistant enetrococci. Transposon 1546 (Tn1546) harboring the van A gene cluster, plays an important role in the horizontal transfer of van A gene. In this study, we examined the phenotypic and genotypic diversity of a number of clinical VRE.Materials and Methods: Twenty-four clinical VRE isolated from two university hospitals in Tehran were examined based on their antimicrobial susceptibility, TN1546 related element organization and pulsed-field gel electrophoresis (PFGE) patterns. Integration of well-studied insertion sequence elements IS1216V, IS1542 and IS1251 was examined by PCR mapping and sequencing.Results: From 24 isolates, 15 isolates with Van A phenotype and 9 isolates with Van B phenotype were identified which both groups interestingly possessed the van A gene. According to PCR mapping, our isolates were assigned to 6 main groups. In 14 (58.3%) isolates, IS1216V was inserted in vanX-vanY region and/or in truncated left-hand of TN1546-like elements. In 11 (45.8%) isolates, both IS1216V and IS1542 were inserted in van X-van Y and orf2-vanR regions, respectively and none of them harbored IS1251. Interestingly, PFGE of the isolates showed a high degree of diversity.Conclusion: PCR mapping revealed that Van A elements in our isolates were highly heterogeneous. Overall, we found no correlation between transposon type and PFGE pattern. Genetic diversity of VRE provides practical information for epidemiological studies and our data showed horizontal transfer of VRE in this region.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    OSKOUEI, MAHVASH, & FAROKH, P.. (2010). DISTRIBUTION OF INSERTION SEQUENCES ASSOCIATED WITH TN1546 AND CLONAL DIVERSITY OF VANCOMYCIN-RESISTANT ENTEROCOCCI ISOLATED FROM PATIENTS IN TEHRAN, IRAN. IRANIAN JOURNAL OF MICROBIOLOGY, 2(1), 15-22. SID. https://sid.ir/paper/315234/en

    Vancouver: Copy

    OSKOUEI MAHVASH, FAROKH P.. DISTRIBUTION OF INSERTION SEQUENCES ASSOCIATED WITH TN1546 AND CLONAL DIVERSITY OF VANCOMYCIN-RESISTANT ENTEROCOCCI ISOLATED FROM PATIENTS IN TEHRAN, IRAN. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2010;2(1):15-22. Available from: https://sid.ir/paper/315234/en

    IEEE: Copy

    MAHVASH OSKOUEI, and P. FAROKH, “DISTRIBUTION OF INSERTION SEQUENCES ASSOCIATED WITH TN1546 AND CLONAL DIVERSITY OF VANCOMYCIN-RESISTANT ENTEROCOCCI ISOLATED FROM PATIENTS IN TEHRAN, IRAN,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 2, no. 1, pp. 15–22, 2010, [Online]. Available: https://sid.ir/paper/315234/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top