مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

280
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

98
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

OPTIMIZATION AND COMPARATIVE CHARACTERIZATION OF NEURAMINIDASE ACTIVITIES FROM PSEUDOMONAS AERUGINOSA WITH KLEBSIELLA PNEUMONIAE, HEP-2 CELL, SHEEP KIDNEY AND RAT LIVER LYSOSOME

Pages

  33-40

Abstract

 Background and Objectives: The properties of NEURAMINIDASE produced by P. aeruginosa strain PAO1 during growth in a defined medium (BHI) was examined and compared with some NEURAMINIDASE features of K. pneumoniae in this investigation.Materials and Methods: The enzyme was isolated from concentrated culture supernatants of P. aeruginosa which was used in a sensitive FLUOROMETRIC ASSAY by using 2’-(4-methylumbelliferyl) a-D-N acetylneuraminic acid as substrate.Results: NEURAMINIDASE production in P. AERUGINOSA PAO1 paralleled bacterial growth in defined medium (BHI) and was maximal in the late logarithmic phase of growth but decreased during the stationary phase, probably owing to protease production or thermal instability. Highest production of P. AERUGINOSA PAO1 NEURAMINIDASE was in BHI culture media. The NEURAMINIDASE of P. AERUGINOSA PAO1 possessed an optimum temperature of activity at 56oC and the activity was maximal at pH 5. Heating the enzyme to 56oC for 45 min., in the presence of bovine serum albumin destroyed 33.1% of it’s activity and addition of Ca+2, EDTA and NANA also decreased activity markedly.Conclusion: The results revealed that the highest SPECIFIC ACTIVITY is for P. AERUGINOSA PAO1.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    GHAZAEI, S., AHMADI, M., & HOSSEINI JAZANI, N.. (2010). OPTIMIZATION AND COMPARATIVE CHARACTERIZATION OF NEURAMINIDASE ACTIVITIES FROM PSEUDOMONAS AERUGINOSA WITH KLEBSIELLA PNEUMONIAE, HEP-2 CELL, SHEEP KIDNEY AND RAT LIVER LYSOSOME. IRANIAN JOURNAL OF MICROBIOLOGY, 2(1), 33-40. SID. https://sid.ir/paper/315237/en

    Vancouver: Copy

    GHAZAEI S., AHMADI M., HOSSEINI JAZANI N.. OPTIMIZATION AND COMPARATIVE CHARACTERIZATION OF NEURAMINIDASE ACTIVITIES FROM PSEUDOMONAS AERUGINOSA WITH KLEBSIELLA PNEUMONIAE, HEP-2 CELL, SHEEP KIDNEY AND RAT LIVER LYSOSOME. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2010;2(1):33-40. Available from: https://sid.ir/paper/315237/en

    IEEE: Copy

    S. GHAZAEI, M. AHMADI, and N. HOSSEINI JAZANI, “OPTIMIZATION AND COMPARATIVE CHARACTERIZATION OF NEURAMINIDASE ACTIVITIES FROM PSEUDOMONAS AERUGINOSA WITH KLEBSIELLA PNEUMONIAE, HEP-2 CELL, SHEEP KIDNEY AND RAT LIVER LYSOSOME,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 2, no. 1, pp. 33–40, 2010, [Online]. Available: https://sid.ir/paper/315237/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button