مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

297
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

100
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DIRECT DETECTION AND SEROGROUP CHARACTERIZATION OF NEISSERIA MENINGITIDIS FROM OUTBREAK OF MENINGOCOCCAL MENINGITIS IN DELHI

Pages

  73-79

Abstract

 Background and Objectives: Rapid clinical manifestation/progression of the meningococcal meningitis and lacunae in conventional bacteriological test often encourages indiscriminate use of antibiotics much before the etiology is established. Accordingly this study was planned to evaluate ctrA PCR for rapid molecular detection. In addition, multiplex PCR and sequencing was done for serogroup prediction to provide essential epidemiological and laboratory evidence for decision makers of health department of the country for choosing appropriate vaccine and phylogenetic analysis to establish its lineage. Materials and Methods: 73 CSF samples, collected from equal number of suspected cases, were investigated by both bacteriological (microscopy, culture, LA and drug sensitivity testing) as well as molecular tests i.e. PCR targeting conserved ctrA gene, multiplex PCR for serogroup characterization and DNA sequencing.Results: ctrA PCR revealed sensitivity, specificity, positive predictive value and negative predictive values of 93.15%, 100%, 100%, and 88.23% respectively. Multiplex PCR based genogrouping followed by DNA sequencing, BLAST and phylogenetic analysis revealed complete homology with earlier submitted NEISSERIA MENINGITIDIS serogroup A strain Z2491 to suggest the sole involvement of only serogroup A in the outbreak. Two strains showed resistance to cefuroxime, ciprofloxacin, nalidixic acid. Only one strain showed resistance to ciprofloxacin, emphasizing the need for a constant surveillance system. Conclusion: These diagnostic molecular tools are of paramount importance in establishing etiology, serogrouping, and epidemiological surveillance especially in developing countries like India.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    NEGI, S.S., GROVER, S.S., RAUTELA, S.S., RAWAT, D.S., GUPTA, S., KHARE, S., LAL, S., & RAI, A.. (2010). DIRECT DETECTION AND SEROGROUP CHARACTERIZATION OF NEISSERIA MENINGITIDIS FROM OUTBREAK OF MENINGOCOCCAL MENINGITIS IN DELHI. IRANIAN JOURNAL OF MICROBIOLOGY, 2(2), 73-79. SID. https://sid.ir/paper/315246/en

    Vancouver: Copy

    NEGI S.S., GROVER S.S., RAUTELA S.S., RAWAT D.S., GUPTA S., KHARE S., LAL S., RAI A.. DIRECT DETECTION AND SEROGROUP CHARACTERIZATION OF NEISSERIA MENINGITIDIS FROM OUTBREAK OF MENINGOCOCCAL MENINGITIS IN DELHI. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2010;2(2):73-79. Available from: https://sid.ir/paper/315246/en

    IEEE: Copy

    S.S. NEGI, S.S. GROVER, S.S. RAUTELA, D.S. RAWAT, S. GUPTA, S. KHARE, S. LAL, and A. RAI, “DIRECT DETECTION AND SEROGROUP CHARACTERIZATION OF NEISSERIA MENINGITIDIS FROM OUTBREAK OF MENINGOCOCCAL MENINGITIS IN DELHI,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 2, no. 2, pp. 73–79, 2010, [Online]. Available: https://sid.ir/paper/315246/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button