DEVELOPMENT OF A NEW DNA EXTRACTION PROTOCOL FOR PFGE TYPING OF MYCOBACTERIUM TUBERCULOSIS COMPLEX

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GHODOUSI A.; VATANI S.; DARBAN SAROKHALIL D.; OMRANI M.; FOULADI A.A.; KHOSARAVI A.D.; FEYZABADI M.M.

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Journal Paper

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Journal: Iranian Journal of Microbiology Year:2012 | Volume:4 | Issue:1 Page(s): 43-45

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Title

DEVELOPMENT OF A NEW DNA EXTRACTION PROTOCOL FOR PFGE TYPING OF MYCOBACTERIUM TUBERCULOSIS COMPLEX

Author(s)

Keywords

MYCOBACTERIUM TUBERCULOSIS

TB-COMPLEX

PULSED-FILED GEL ELECTROPHORESIS

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of MYCOBACTERIUM TUBERCULOSIS complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of MYCOBACTERIUM TUBERCULOSIS complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

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APA: Copy

GHODOUSI, A., VATANI, S., DARBAN SAROKHALIL, D., OMRANI, M., FOULADI, A.A., KHOSARAVI, A.D., & FEYZABADI, M.M.. (2012). DEVELOPMENT OF A NEW DNA EXTRACTION PROTOCOL FOR PFGE TYPING OF MYCOBACTERIUM TUBERCULOSIS COMPLEX. IRANIAN JOURNAL OF MICROBIOLOGY, 4(1), 43-45. SID. https://sid.ir/paper/315303/en

Vancouver: Copy

GHODOUSI A., VATANI S., DARBAN SAROKHALIL D., OMRANI M., FOULADI A.A., KHOSARAVI A.D., FEYZABADI M.M.. DEVELOPMENT OF A NEW DNA EXTRACTION PROTOCOL FOR PFGE TYPING OF MYCOBACTERIUM TUBERCULOSIS COMPLEX. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2012;4(1):43-45. Available from: https://sid.ir/paper/315303/en

IEEE: Copy

A. GHODOUSI, S. VATANI, D. DARBAN SAROKHALIL, M. OMRANI, A.A. FOULADI, A.D. KHOSARAVI, and M.M. FEYZABADI, “DEVELOPMENT OF A NEW DNA EXTRACTION PROTOCOL FOR PFGE TYPING OF MYCOBACTERIUM TUBERCULOSIS COMPLEX,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 4, no. 1, pp. 43–45, 2012, [Online]. Available: https://sid.ir/paper/315303/en

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