CLONING AND EXPRESSION OF CLOSTRIDIUM PERFRINGENS TYPE D VACCINE STRAIN EPSILON TOXIN GENE IN E. COLI AS A RECOMBINANT VACCINE CANDIDATE

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AZIMINIA PARASTOO; PILEHCHIAN LANGROUDI REZA; ESMAEILNIA KASRA

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Journal: Iranian Journal of Microbiology Year:2016 | Volume:8 | Issue:4 Page(s): 0-0

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Title

CLONING AND EXPRESSION OF CLOSTRIDIUM PERFRINGENS TYPE D VACCINE STRAIN EPSILON TOXIN GENE IN E. COLI AS A RECOMBINANT VACCINE CANDIDATE

Author(s)

AZIMINIA PARASTOO | PILEHCHIAN LANGROUDI REZA | ESMAEILNIA KASRA | Issue Writer Certificate

Keywords

C. PERFRINGENS

EPSILON TOXIN GENE

VACCINE STRAIN

CLONING

EXPRESSION

Abstract

Background and Objectives: Clostridium perfringens, a Gram-positive obligate anaerobic bacterium, is able to form resistantspores which are widely distributed in the environment. C. PERFRINGENS is subdivided into five types A to E based on itsfour major alpha, beta, epsilon and iota toxins. The aim of the present study was CLONING and EXPRESSION of C. PERFRINGENStype D VACCINE STRAIN EPSILON TOXIN GENE.Materials and Methods: Genomic DNA was extracted and the EPSILON TOXIN GENE was amplified using Pfu DNA polymerase.The PCR product was cloned into pJET1.2/blunt CLONING vector. The recombinant vector (pJETε) was sequenced usinguniversal primers. At the next step EPSILON TOXIN GENE was subcloned into pET22b (+) EXPRESSION vector and transformedinto E. coli Rosetta (DE3) host strain.Results: The recombinant protein has been expressed in E. coli Rosetta (DE3) cells after subcloning of C. PERFRINGENS etxgene (1008 bp) into the EXPRESSION vector.Conclusion: We concluded that E. coli Rosetta strain was suitable for the EXPRESSION of recombinant C. PERFRINGENS epsilontoxin protein from pET22ε EXPRESSION vector. This recombinant cell can be used for further research on recombinant vaccinedevelopment.

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APA: Copy

AZIMINIA, PARASTOO, PILEHCHIAN LANGROUDI, REZA, & ESMAEILNIA, KASRA. (2016). CLONING AND EXPRESSION OF CLOSTRIDIUM PERFRINGENS TYPE D VACCINE STRAIN EPSILON TOXIN GENE IN E. COLI AS A RECOMBINANT VACCINE CANDIDATE. IRANIAN JOURNAL OF MICROBIOLOGY, 8(4), 0-0. SID. https://sid.ir/paper/315401/en

Vancouver: Copy

AZIMINIA PARASTOO, PILEHCHIAN LANGROUDI REZA, ESMAEILNIA KASRA. CLONING AND EXPRESSION OF CLOSTRIDIUM PERFRINGENS TYPE D VACCINE STRAIN EPSILON TOXIN GENE IN E. COLI AS A RECOMBINANT VACCINE CANDIDATE. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2016;8(4):0-0. Available from: https://sid.ir/paper/315401/en

IEEE: Copy

PARASTOO AZIMINIA, REZA PILEHCHIAN LANGROUDI, and KASRA ESMAEILNIA, “CLONING AND EXPRESSION OF CLOSTRIDIUM PERFRINGENS TYPE D VACCINE STRAIN EPSILON TOXIN GENE IN E. COLI AS A RECOMBINANT VACCINE CANDIDATE,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 8, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/315401/en

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