INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE

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HOSSEINI SEYEDEH MARZIEH; TABARAEI BAHMAN; HADADIAN SHAHIN; YAVARI FERESHTEH; KAMALIJAMIL RAZIEH; SHAYESTEHPOUR MOHAMMAD; NEJATI MAHDI; VAEZ JAVAD

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Journal: Archives of Advances in Biosciences Year:2011 | Volume:2 | Issue:3 Page(s): 24-29

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Title

INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE

Author(s)

Keywords

HOST CELL PROTEIN (HCP)

RECOMBINANT HEPATITIS B VIRUS (HBV) VACCINE

CONTAMINATION

Abstract

One of the most important aspects in recombinant biologic production, based on GMP rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules CONTAMINATION rate in final product. The purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. It is possible that the final product contaminated to the host cell protein during purification stages of HBsAg (HBV vaccine). The protein purification costs depend on the purification procedures required. Nowadays several companies produce commercial kits for identification and assessment of host cell protein CONTAMINATION based on ELISA and Western blotting methods. But high prices, difference in sensitivity and lack of easy access to these kits sometimes create problems. So, in this study, two methods of Ammonium sulphate and caprilic acid precipitation technique were used separately for IgG purification. The results showed that IgG purification increased by 97% in caprylic acid method, compared with only a 77% increase in ammonium sulphate method. There were also significant differences in specificity and sensitivity between our standardized ELISA technique and using commercial kit (Cygnus CHO HCP).

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APA: Copy

HOSSEINI, SEYEDEH MARZIEH, TABARAEI, BAHMAN, HADADIAN, SHAHIN, YAVARI, FERESHTEH, KAMALIJAMIL, RAZIEH, SHAYESTEHPOUR, MOHAMMAD, NEJATI, MAHDI, & VAEZ, JAVAD. (2011). INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), 2(3), 24-29. SID. https://sid.ir/paper/315786/en

Vancouver: Copy

HOSSEINI SEYEDEH MARZIEH, TABARAEI BAHMAN, HADADIAN SHAHIN, YAVARI FERESHTEH, KAMALIJAMIL RAZIEH, SHAYESTEHPOUR MOHAMMAD, NEJATI MAHDI, VAEZ JAVAD. INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES)[Internet]. 2011;2(3):24-29. Available from: https://sid.ir/paper/315786/en

IEEE: Copy

SEYEDEH MARZIEH HOSSEINI, BAHMAN TABARAEI, SHAHIN HADADIAN, FERESHTEH YAVARI, RAZIEH KAMALIJAMIL, MOHAMMAD SHAYESTEHPOUR, MAHDI NEJATI, and JAVAD VAEZ, “INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE,” ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), vol. 2, no. 3, pp. 24–29, 2011, [Online]. Available: https://sid.ir/paper/315786/en

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