مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

478
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

171
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

HETEROLOGOUS EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN OIL SEEDS OF BRASSICA NAPUS L.

Pages

  1-9

Abstract

 Background: Hepatitis c virus (HCV), prevalent among 3% of the world population, is a major worldwide public health concern and an effective vaccination could help to overcome this problem. Plant seeds as low-cost vaccine expression platforms are highly desirable to produce antigens.Objectives: The present study was aimed at investigating the possible expression of recombinant HCV CORE PROTEIN, as a leading HCV vaccine candidate, in canola (BRASSICA NAPUS) plant seeds in order to be used as an effective immunogen for vaccine researches.Materials and Methods: A codon-optimized gene harboring the Kozak sequence, 6 × His-tag, HCVcp (1 - 122 residues) and KDEL (Lys-Asp-Glu-Leu) peptide in tandem was designed and expressed under the control of the seed specific promoter, fatty acid elongase 1 (FAE1), to accumulate the recombinant protein in canola (B. napus L.) seeds. Transgenic lines were screened and the presence of the transgene was confirmed in the T0 plants by polymerase chain reaction (PCR). The quantity and quality of the HCV CORE PROTEIN (HCVcp) in transgenic seeds were evaluated by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively.Results: Western blot analysis using anti-His antibody confirmed the presence of a 15 kDa protein in the seeds of T1 transgenic lines. The amount of antigenic protein accumulated in the seeds of these transgenic lines was up to 0.05% of the total soluble protein (TSP).Conclusions: The canola oilseeds could provide a useful expression system to produce HCV CORE PROTEIN as a vaccine candidate.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    MOHAMMADZADEH, SARA, ROOHVAND, FARZIN, AJDARY, SOHEILA, EHSANI, PARASTOO, & HATEF SALMANIAN, ALI. (2015). HETEROLOGOUS EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN OIL SEEDS OF BRASSICA NAPUS L.. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(11), 1-9. SID. https://sid.ir/paper/317443/en

    Vancouver: Copy

    MOHAMMADZADEH SARA, ROOHVAND FARZIN, AJDARY SOHEILA, EHSANI PARASTOO, HATEF SALMANIAN ALI. HETEROLOGOUS EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN OIL SEEDS OF BRASSICA NAPUS L.. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(11):1-9. Available from: https://sid.ir/paper/317443/en

    IEEE: Copy

    SARA MOHAMMADZADEH, FARZIN ROOHVAND, SOHEILA AJDARY, PARASTOO EHSANI, and ALI HATEF SALMANIAN, “HETEROLOGOUS EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN OIL SEEDS OF BRASSICA NAPUS L.,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 11, pp. 1–9, 2015, [Online]. Available: https://sid.ir/paper/317443/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button