COMPARATIVE PROTEOMICS ANALYSIS OF TWO STRAINS OF NEISSERIA MENINGITIDIS SEROGROUP B AND NEISSERIA LACTAMICA

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SHEIKHI RAHELEH; AMIN MANSOUR; HAMIDINIA MARYAM; ASSAREHZADEGAN MOHAMMAD ALI; ROSTAMI SOODABEH; MOJTAHEDI ZAHRA

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:11 Page(s): 1-9

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Title

COMPARATIVE PROTEOMICS ANALYSIS OF TWO STRAINS OF NEISSERIA MENINGITIDIS SEROGROUP B AND NEISSERIA LACTAMICA

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Keywords

PROTEOMICS

MASS SPECTROMETRY

TWO-DIMENSIONAL GEL ELECTROPHORESIS

NEISSERIA MENINGITIDIS SEROGROUP B

NEISSERIA LACTAMICA

Abstract

Background: Antigenic similarities between NEISSERIA LACTAMICA as a commensal species and N. meningitidis serogroup B (NmB) as an important cause of meningitis infection have been considered for the development of an effective vaccine based on their common proteins to prevent life-threatening bacterial meningitis.Objectives: The main aims of this study were to determine whole proteome profiles of N. lactamica strains and to compare them with whole proteome profile of a reference strain of NmB for identification of some of common proteins between the two species.Materials and Methods: We compared the whole proteomic profiles of N. lactamica strains and a reference strain of NmB. Lysates from bacterial strains were resolved by TWO-DIMENSIONAL GEL ELECTROPHORESIS (2-DE), followed by Coomassie Brilliant blue staining. Some of the protein spots were excised from the gel and subjected to matrix-assisted laser desorption/ionization-tandem time-of-flight MASS SPECTROMETRY (MALDI-TOF/TOF MS) analysis.Results: The analysis of Coomassie-stained gels using ImageMaster 2D Platinum software identified approximately 800 reproducible protein spots in the range of pI 4.5 - 9.5 and Mr of 8 - 100 kDa for each 2-DE gel of the studied bacterial strains. By comparing proteome maps of 2-DE gels, more than 200 common protein spots were recognized between the two species. Forty-eight common protein spots between the studied bacterial strains were identified by MALDI-TOF/TOF-MS. The results indicated that among the protein spots identified by MOLDI-TOF/TOF MASS SPECTROMETRY, the groups of proteins included cell surface, energy metabolism, amino acid transport and metabolism, coenzyme metabolism, defense, multifunctional cellular processes, DNA, RNA and protein synthesis, ribosomal structure, regulatory functions, replication, transcription, translation, unknown and hypothetical proteins with unknown function. We found that N. lactamica strains have a proteome profile somewhat similar to each other and slightly different with NmB.Conclusions: These results show the usefulness of proteome analysis in successful identification of the common proteins between N. lactamica strains and NmB. This PROTEOMICS analysis is the starting point in the path of knowledge development about whole proteome profiles of N. lactamica strains.

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APA: Copy

SHEIKHI, RAHELEH, AMIN, MANSOUR, HAMIDINIA, MARYAM, ASSAREHZADEGAN, MOHAMMAD ALI, ROSTAMI, SOODABEH, & MOJTAHEDI, ZAHRA. (2015). COMPARATIVE PROTEOMICS ANALYSIS OF TWO STRAINS OF NEISSERIA MENINGITIDIS SEROGROUP B AND NEISSERIA LACTAMICA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(11), 1-9. SID. https://sid.ir/paper/317445/en

Vancouver: Copy

SHEIKHI RAHELEH, AMIN MANSOUR, HAMIDINIA MARYAM, ASSAREHZADEGAN MOHAMMAD ALI, ROSTAMI SOODABEH, MOJTAHEDI ZAHRA. COMPARATIVE PROTEOMICS ANALYSIS OF TWO STRAINS OF NEISSERIA MENINGITIDIS SEROGROUP B AND NEISSERIA LACTAMICA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(11):1-9. Available from: https://sid.ir/paper/317445/en

IEEE: Copy

RAHELEH SHEIKHI, MANSOUR AMIN, MARYAM HAMIDINIA, MOHAMMAD ALI ASSAREHZADEGAN, SOODABEH ROSTAMI, and ZAHRA MOJTAHEDI, “COMPARATIVE PROTEOMICS ANALYSIS OF TWO STRAINS OF NEISSERIA MENINGITIDIS SEROGROUP B AND NEISSERIA LACTAMICA,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 11, pp. 1–9, 2015, [Online]. Available: https://sid.ir/paper/317445/en

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