ANALYSIS OF BACILLUS THURINGIENSIS POPULATION DYNAMICS AND ITS INTERACTION WITH PSEUDOMONAS FLUORESCENS IN SOIL

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ROJAS RUIZ NORMA ELENA; SANSINENEA ROYANO ESTIBALIZ; CEDILLO RAMIREZ MARIA LILIA; MARSCH MORENO RODOLFO; SANCHEZ ALONSO PATRICIA; VAZQUEZ CRUZ CANDELARIO

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:9 Page(s): 1-7

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Title

ANALYSIS OF BACILLUS THURINGIENSIS POPULATION DYNAMICS AND ITS INTERACTION WITH PSEUDOMONAS FLUORESCENS IN SOIL

Author(s)

Keywords

MICROBIAL INTERACTIONS

ANTIBIOSIS

SPORES

BACILLUS THURINGIENSIS

PSEUDOMONAS FLUORESCENS

Abstract

Background: BACILLUS THURINGIENSIS is the most successful biological control agent, however, studies so far have shown that B. thuringiensis is very sensitive to environmental factors such as soil moisture and pH. Ultraviolet light from the sun had been considered as the main limiting factor for its persistence in soil and it has recently been shown that the antagonism exerted by other native soil organisms, such as PSEUDOMONAS FLUORESCENS, is a determining factor in the persistence of this bacterium under in vitro culture conditions.Objectives: The aim of the present investigation was to analyze the population dynamics of B. thuringiensis and its interaction with P. fluorescens using microbiological and molecular methods in soil, under different conditions, and to determinate the effect of nutrients and moisture on its interaction.Materials and Methods: The monitoring was performed by microbiological methods, such as viable count of bacteria, and molecular methods such as Polymerase Chain Reaction (PCR) and hybridization, using the direct extraction of DNA from populations of inoculated soil.Results: The analysis of the interaction between B. thuringiensis and P. fluorescens in soil indicated that the disappearance of B. thuringiensis IPS82 is not dependent on the moisture but the composition of nutrients that may be affecting the secretion of toxic compounds in the environment of P. fluorescens. The results showed that the recovered cells were mostly SPORES and not vegetative cells in all proved treatments. The molecular methods were effective for monitoring bacterial population inoculated in soil.Conclusions: BACILLUS THURINGIENSIS is very sensitive to the interaction of P. fluorescens, however is capable to survive in soil due to its capacity of sporulate. Some of the cells in the form of SPORES germinated and folded slightly and remained in a constant cycle of sporulation and germination. This confirms that B. thuringiensis IPS82 can germinate, grow and sporulate in soil.

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APA: Copy

ROJAS RUIZ, NORMA ELENA, SANSINENEA ROYANO, ESTIBALIZ, CEDILLO RAMIREZ, MARIA LILIA, MARSCH MORENO, RODOLFO, SANCHEZ ALONSO, PATRICIA, & VAZQUEZ CRUZ, CANDELARIO. (2015). ANALYSIS OF BACILLUS THURINGIENSIS POPULATION DYNAMICS AND ITS INTERACTION WITH PSEUDOMONAS FLUORESCENS IN SOIL. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(9), 1-7. SID. https://sid.ir/paper/317488/en

Vancouver: Copy

ROJAS RUIZ NORMA ELENA, SANSINENEA ROYANO ESTIBALIZ, CEDILLO RAMIREZ MARIA LILIA, MARSCH MORENO RODOLFO, SANCHEZ ALONSO PATRICIA, VAZQUEZ CRUZ CANDELARIO. ANALYSIS OF BACILLUS THURINGIENSIS POPULATION DYNAMICS AND ITS INTERACTION WITH PSEUDOMONAS FLUORESCENS IN SOIL. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(9):1-7. Available from: https://sid.ir/paper/317488/en

IEEE: Copy

NORMA ELENA ROJAS RUIZ, ESTIBALIZ SANSINENEA ROYANO, MARIA LILIA CEDILLO RAMIREZ, RODOLFO MARSCH MORENO, PATRICIA SANCHEZ ALONSO, and CANDELARIO VAZQUEZ CRUZ, “ANALYSIS OF BACILLUS THURINGIENSIS POPULATION DYNAMICS AND ITS INTERACTION WITH PSEUDOMONAS FLUORESCENS IN SOIL,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 9, pp. 1–7, 2015, [Online]. Available: https://sid.ir/paper/317488/en

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