مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

MULTIPLEX SYBR GREEN REAL-TIME PCR ASSAY FOR DETECTION OF RESPIRATORY VIRUSES

Pages

  1-5

Abstract

 Background: It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.Objectives: The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR GREEN real-time polymerase chain reaction (PCR).Materials and Methods: Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR GREEN multiplex REAL-TIME PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43.Results: Application of the multiplex SYBR GREEN REAL-TIME PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012).Conclusions: Multiplex SYBR GREEN REAL-TIME PCR is a rapid and relatively inexpensive method for detection of respiratory viruses.

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    APA: Copy

    SULTANI, MOZHDEH, MOKHTARI AZAD, TALAT, ESHRAGIAN, MOHAMMADREZA, SHADAB, AZADEH, NASERI, MARYAM, EILAMI, OWRANG, & YAVARIAN, JILA. (2015). MULTIPLEX SYBR GREEN REAL-TIME PCR ASSAY FOR DETECTION OF RESPIRATORY VIRUSES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(8), 1-5. SID. https://sid.ir/paper/317498/en

    Vancouver: Copy

    SULTANI MOZHDEH, MOKHTARI AZAD TALAT, ESHRAGIAN MOHAMMADREZA, SHADAB AZADEH, NASERI MARYAM, EILAMI OWRANG, YAVARIAN JILA. MULTIPLEX SYBR GREEN REAL-TIME PCR ASSAY FOR DETECTION OF RESPIRATORY VIRUSES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(8):1-5. Available from: https://sid.ir/paper/317498/en

    IEEE: Copy

    MOZHDEH SULTANI, TALAT MOKHTARI AZAD, MOHAMMADREZA ESHRAGIAN, AZADEH SHADAB, MARYAM NASERI, OWRANG EILAMI, and JILA YAVARIAN, “MULTIPLEX SYBR GREEN REAL-TIME PCR ASSAY FOR DETECTION OF RESPIRATORY VIRUSES,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 8, pp. 1–5, 2015, [Online]. Available: https://sid.ir/paper/317498/en

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