MOLECULAR IDENTIFICATION OF RESISTANCE DETERMINANTS, INTEGRONS AND GENETIC RELATEDNESS OF EXTENSIVELY DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALS IN TEHRAN, IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAJAR PEERAYEH SHAHIN; KARMOSTAJI AFSANEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:7 Page(s): 0-0

Download Full-Text

View:

359

Download:

151

Cites:

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF RESISTANCE DETERMINANTS, INTEGRONS AND GENETIC RELATEDNESS OF EXTENSIVELY DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALS IN TEHRAN, IRAN

Author(s)

NAJAR PEERAYEH SHAHIN | KARMOSTAJI AFSANEH | Issue Writer Certificate

Keywords

EXTENSIVELY DRUG-RESISTANT

INTEGRONS

OXACILLINASE

ACINETOBACTER BAUMANNII

Abstract

Background: ACINETOBACTER BAUMANNII has emerged as an important nosocomial pathogen. Hospital outbreaks of extensively drug resistant (XDR) A. baumannii are a great concern.Objectives: Aims of this study were to characterize the resistance determinants and genetic relatedness of (XDR) A. baumannii isolates in hospitals in Tehran, Iran.Materials and Methods: During a three-year study, clinical isolates of A. baumannii were collected from two hospitals in Tehran, Iran. Susceptibility testing to antibiotics was performed by disk diffusion method and XDR A. baumannii isolates were identified. Genes’ encoding for carbapenemase production and INTEGRONS were identified by PCR. MICs of imipenem and meropenem were determined by agar dilution. Multiple locus variable-number tandem repeat analysis (MLVA) typing was used to determine genetic relationships of XDR isolates.Results: Using PCR for amplification of blaOXA-51, 93.9% (123.131) of isolates were identified as A. baumannii and 24.4% (30.123) were XDR. These isolates were resistant to gentamicin, ciprofloxacin, amikacin, cotrimoxazole, cefepime, cefotaxime, aztreonam and ceftazidime. Thirty percent of the isolates were resistant to tigecycline. All isolates were susceptible to colistin and polymyxin-B, while 93.3% (28.30) possessed blaOXA-23-like and 6.7% (2.30) possessed blaOXA-24-like. All isolates possessed insertion sequence (ISAba1) in the upstream region of the OXA-23-like gene. Almost 96.7% (29.30) of the isolates were positive for class I integron and 43.3% (13.30) for class II. These isolates were also positive for class I. Class III integron was not detected. MLVA typing of XDR isolates showed seven clonally complexes and 16 singletons.Conclusions: The population structure of the A. baumannii isolates in our hospitals was genetically diverse. A significant association between XDR pattern and presence of class 1 integron (P<0.001) was found indicating that many antibiotic resistance determinants are involved in development of XDR strains.

Cites

Prevalence of integron classes in Gram-negative clinical isolated bacteria in Iran: a systematic review and meta-analysis

References

Cite

APA: Copy

NAJAR PEERAYEH, SHAHIN, & KARMOSTAJI, AFSANEH. (2015). MOLECULAR IDENTIFICATION OF RESISTANCE DETERMINANTS, INTEGRONS AND GENETIC RELATEDNESS OF EXTENSIVELY DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALS IN TEHRAN, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(7), 0-0. SID. https://sid.ir/paper/317521/en

Vancouver: Copy

NAJAR PEERAYEH SHAHIN, KARMOSTAJI AFSANEH. MOLECULAR IDENTIFICATION OF RESISTANCE DETERMINANTS, INTEGRONS AND GENETIC RELATEDNESS OF EXTENSIVELY DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALS IN TEHRAN, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(7):0-0. Available from: https://sid.ir/paper/317521/en

IEEE: Copy

SHAHIN NAJAR PEERAYEH, and AFSANEH KARMOSTAJI, “MOLECULAR IDENTIFICATION OF RESISTANCE DETERMINANTS, INTEGRONS AND GENETIC RELATEDNESS OF EXTENSIVELY DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALS IN TEHRAN, IRAN,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 7, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/317521/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops