مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PSEUDOMONAS AERUGINOSA PAO-1 LIPOPOLYSACCHARIDE-DIPHTHERIA TOXOID CONJUGATE VACCINE: PREPARATION, CHARACTERIZATION AND IMMUNOGENICITY

Pages

  0-0

Abstract

 Background: Treatment of PSEUDOMONAS AERUGINOSA PAO-1 infections through immunological means has been proved to be efficient and protective.Objectives: The purpose of this study was to produce a CONJUGATE vaccine composed of detoxified lipopolysaccharide (D-LPS) P. aeruginosa and DIPHTHERIA TOXOID (DT).Materials and Methods: Firstly, LPS was purified and characterized from P. aeruginosa PAO1 and then detoxified. D-LPS was covalently coupled to DT as a carrier protein via amidation method with adipic acid dihydrazide (ADH) as a spacer molecule and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDAC) as a linker. The molar ratio of LPS to DT in the prepared CONJUGATE was 3: 1. The immunogenicity of D-LPS-DT CONJUGATE vaccine in mice model was evaluated as well.Results: The CONJUGATE was devoid of endotoxin activity and 0.125 U/mL of D-LPS was acceptable for immunization. D-LPS-DT CONJUGATE was nonpyrogenic for rabbits and nontoxic for mice. Mice immunization with D-LPS-DT CONJUGATE vaccine elicited the fourfold higher IgG antibody compared to D-LPS. Anti-LPS IgG antibody was predominantly IgG1 subclass and then IgG3, IgG2a and IgG2b, respectively.Conclusions: Vaccine based on the conjugation of P. aeruginosa PAO-1 LPS with DT increased anti-LPS antibodies and had a significant potential to protect against Pseudomonas infections.

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    APA: Copy

    NAJAFZADEH, FAEZEH, SHAPOURI, REZA, RAHNEMA, MEHDI, ROKHSARTALAB AZAR, SHADI, & KIANMEHR, ANVARSADAT. (2015). PSEUDOMONAS AERUGINOSA PAO-1 LIPOPOLYSACCHARIDE-DIPHTHERIA TOXOID CONJUGATE VACCINE: PREPARATION, CHARACTERIZATION AND IMMUNOGENICITY. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(6), 0-0. SID. https://sid.ir/paper/317529/en

    Vancouver: Copy

    NAJAFZADEH FAEZEH, SHAPOURI REZA, RAHNEMA MEHDI, ROKHSARTALAB AZAR SHADI, KIANMEHR ANVARSADAT. PSEUDOMONAS AERUGINOSA PAO-1 LIPOPOLYSACCHARIDE-DIPHTHERIA TOXOID CONJUGATE VACCINE: PREPARATION, CHARACTERIZATION AND IMMUNOGENICITY. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(6):0-0. Available from: https://sid.ir/paper/317529/en

    IEEE: Copy

    FAEZEH NAJAFZADEH, REZA SHAPOURI, MEHDI RAHNEMA, SHADI ROKHSARTALAB AZAR, and ANVARSADAT KIANMEHR, “PSEUDOMONAS AERUGINOSA PAO-1 LIPOPOLYSACCHARIDE-DIPHTHERIA TOXOID CONJUGATE VACCINE: PREPARATION, CHARACTERIZATION AND IMMUNOGENICITY,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 6, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/317529/en

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