GENETIC CHARACTERIZATION OF CAMPYLOBACTER JEJUNI AND C. COLI ISOLATED FROM BROILERS USING FLAA PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD IN SHIRAZ, SOUTHERN IRAN

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KHOSHBAKHT RAHEM; TABATABAEI MOHAMMAD; HOSSEINZADEH SAEID; SHIRZAD ASKI HESAMADDIN; SEIFI SPEED

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:5 Page(s): 0-0

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Title

GENETIC CHARACTERIZATION OF CAMPYLOBACTER JEJUNI AND C. COLI ISOLATED FROM BROILERS USING FLAA PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD IN SHIRAZ, SOUTHERN IRAN

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Keywords

CAMPYLOBACTER JEJUNI

C. COLI

RESTRICTION FRAGMENT LENGTH POLYMORPHISM

IRAN

Abstract

Background: Thermophilic campylobacters, particularly CAMPYLOBACTER JEJUNI and C. COLI are the main agents of human campylobacteriosis. Campylobacter contaminated chicken products is the most important source of foodborne gastroenteritis. Evaluation of genetic diversity among Campylobacter population is critical for understanding the epidemiology of this bacterium and developing effective control strategies against Campylobacter infections and other related disorders.Objectives: The aim of this study was to investigate the polymorphism of thermophilic Campylobacter isolated from broiler fecal samples in Shiraz, southern IRAN.Materials and Methods: Ninety Campylobacter isolates were recovered from broiler feces using enrichment process followed by cultivation method. The isolates were species typing on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two thermophilic species. To evaluate strain diversity of thermophilic Campylobacter isolates, flaA PCR-Restriction Fragment Length Polymorphism (RFLP) was performed using DdeI restriction enzyme.Results: All 90 Campylobacter isolates confirmed by m-PCR were successfully typed using flaA-PCR-RFLP. Eleven different types were defined according to flaA-typing method and the RFLP patterns were located at three separate clusters in RFLP image analysis dendrogram.Conclusions: CAMPYLOBACTER JEJUNI isolates significantly showed more variety than C. COLI isolates. A relatively low genetic diversity existed among C. jejuni and C. COLI isolated from broilers in Shiraz, southern IRAN. In our knowledge, this was the first report of genetic diversity among broiler originated human pathogen thermophilic campylobacters in Shiraz, southern IRAN.

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APA: Copy

KHOSHBAKHT, RAHEM, TABATABAEI, MOHAMMAD, HOSSEINZADEH, SAEID, SHIRZAD ASKI, HESAMADDIN, & SEIFI, SPEED. (2015). GENETIC CHARACTERIZATION OF CAMPYLOBACTER JEJUNI AND C. COLI ISOLATED FROM BROILERS USING FLAA PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD IN SHIRAZ, SOUTHERN IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(5), 0-0. SID. https://sid.ir/paper/317543/en

Vancouver: Copy

KHOSHBAKHT RAHEM, TABATABAEI MOHAMMAD, HOSSEINZADEH SAEID, SHIRZAD ASKI HESAMADDIN, SEIFI SPEED. GENETIC CHARACTERIZATION OF CAMPYLOBACTER JEJUNI AND C. COLI ISOLATED FROM BROILERS USING FLAA PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD IN SHIRAZ, SOUTHERN IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(5):0-0. Available from: https://sid.ir/paper/317543/en

IEEE: Copy

RAHEM KHOSHBAKHT, MOHAMMAD TABATABAEI, SAEID HOSSEINZADEH, HESAMADDIN SHIRZAD ASKI, and SPEED SEIFI, “GENETIC CHARACTERIZATION OF CAMPYLOBACTER JEJUNI AND C. COLI ISOLATED FROM BROILERS USING FLAA PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD IN SHIRAZ, SOUTHERN IRAN,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 5, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/317543/en

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