VIRAL LOAD ANALYSIS OF HEPATITIS C VIRUS IN HUH7.5 CELL CULTURE SYSTEM

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TEIMOURPOUR ROGHAYEH; MESHKAT ZAHRA; GHOLOUBI AIDA; NOMANI HOSEIN; ROSTAMI SINA

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Journal Paper

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:5 Page(s): 0-0

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Title

VIRAL LOAD ANALYSIS OF HEPATITIS C VIRUS IN HUH7.5 CELL CULTURE SYSTEM

Author(s)

Keywords

HEPATITIS C VIRUS

DNA

COMPLEMENTARY

JFH-1

HUH 7.5

REAL-TIME POLYMERASE CHAIN REACTION

Abstract

Background: Previous studies using cell culture systems for the replication of HEPATITIS C VIRUS have opened new research dimensions, and paved the ways for further and detailed studies of the virus in vitro.Objectives: The purpose of the present study was to cultivate HEPATITIS C VIRUS in a cell culture system and evaluate viral amplification.Materials and Methods: In order to propagate HEPATITIS C VIRUS, cloned whole genome of virus, JFH-1, was used. JFH-1 cDNA was introduced into strain JM109 of Escherichia coli and plasmid, containing the viral genome was purified from transformed bacteria. After XbaI digestion, RNA synthesis was induced using T7 RNA polymerase enzyme. Next, eukaryotic cell line HUH 7.5 was transfected by the purified RNA. Finally, Huh-7.5 cell line was infected with replicated virus and viral load was determined using real-time PCR (Polymerase Chain Reaction).Results: The amount of viral load, which was measured using real-time PCR was 17592 IU/mL.Conclusions: In the present study, using cell culture, a high titer (in acceptable range) of infectious HEPATITIS C VIRUS was produced. This method could be used in future studies.

Cites

NANOPARTICLES IN DIAGNOSIS AND TREATMENT OF HEPATITIS C INFECTION: A REVIEW ARTICLE

References

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APA: Copy

TEIMOURPOUR, ROGHAYEH, MESHKAT, ZAHRA, GHOLOUBI, AIDA, NOMANI, HOSEIN, & ROSTAMI, SINA. (2015). VIRAL LOAD ANALYSIS OF HEPATITIS C VIRUS IN HUH7.5 CELL CULTURE SYSTEM. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(5), 0-0. SID. https://sid.ir/paper/317553/en

Vancouver: Copy

TEIMOURPOUR ROGHAYEH, MESHKAT ZAHRA, GHOLOUBI AIDA, NOMANI HOSEIN, ROSTAMI SINA. VIRAL LOAD ANALYSIS OF HEPATITIS C VIRUS IN HUH7.5 CELL CULTURE SYSTEM. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(5):0-0. Available from: https://sid.ir/paper/317553/en

IEEE: Copy

ROGHAYEH TEIMOURPOUR, ZAHRA MESHKAT, AIDA GHOLOUBI, HOSEIN NOMANI, and SINA ROSTAMI, “VIRAL LOAD ANALYSIS OF HEPATITIS C VIRUS IN HUH7.5 CELL CULTURE SYSTEM,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 5, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/317553/en

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