Cytolethal Distending Toxin From Campylobacter jejuni Requires the Cytoskeleton for Toxic Activity

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Mendez Olvera Estela T.; Bustos Martinez Jaime A.; Lopez Vidal Yolanda; Verdugo Rodriguez Antonio; Martinez Gomez Daniel

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:10 Page(s): 0-0

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Title

Cytolethal Distending Toxin From Campylobacter jejuni Requires the Cytoskeleton for Toxic Activity

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Keywords

CDC2 Protein Kinase

Cytolethal Distending Toxin

Cytoskeleton

Latrunculin A

Nocodazole

Campylobacter jejuni

Abstract

Background: Campylobacter jejuni is one of the major causes of infectious diarrhea worldwide. The distending cytolethal toxin (CDT) of Campylobacter spp. interferes with normal cell cycle progression. This toxic effect is considered a result of DNase activity that produces chromosomal DNA damage. To perform this event, the toxin must be endocytosed and translocated to the nucleus. Objectives: The aim of this study was to evaluate the role of the Cytoskeleton in the translocation of CDT to the nucleus. Methods: Campylobacter jejuni ATCC 33291 and seven isolates donated from Instituto de Biotecnologia were used in this study. The presence of CDT genes in C. jejuni strains was determined by PCR. To evaluate the effect of CDT, HeLa cells were treated with bacterial lysate, and the damage and morphological changes were analyzed by microscopy, immunofluorescence staining, and flow cytometry. To evaluate the role of the Cytoskeleton, HeLa cells were treated with either Latrunculin A or by Nocodazole and analyzed by microscopy, flow cytometry, and immunoquantification (ELISA). Results: The results obtained showed that the eight strains of C. jejuni, including the reference strain, had the ability to produce the toxin. Usage of Latrunculin A and Nocodazole, two cytoskeletal inhibitors, blocked the toxic effect in cells treated with the toxin. This phenomenon was evident in flow cytometry analysis and immunoquantification of Cdc2-phosphorylated. Conclusions: This work showed that the cytotoxic activity of the C. jejuni CDT is dependent on its endocytosis. The alteration in the microtubules and actin filaments caused a blockage transit of the toxin, preventing it from reaching the nucleus of the cell, as well as preventing DNA fragmentation and alteration of the cell cycle. The CDT toxin appears to be an important element for the pathogenesis of campylobacteriosis, since all clinical isolates showed the presence of cdtA, cdtB and cdtC genes.

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APA: Copy

Mendez Olvera, Estela T., Bustos Martinez, Jaime A., Lopez Vidal, Yolanda, Verdugo Rodriguez, Antonio, & Martinez Gomez, Daniel. (2016). Cytolethal Distending Toxin From Campylobacter jejuni Requires the Cytoskeleton for Toxic Activity. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(10), 0-0. SID. https://sid.ir/paper/317771/en

Vancouver: Copy

Mendez Olvera Estela T., Bustos Martinez Jaime A., Lopez Vidal Yolanda, Verdugo Rodriguez Antonio, Martinez Gomez Daniel. Cytolethal Distending Toxin From Campylobacter jejuni Requires the Cytoskeleton for Toxic Activity. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(10):0-0. Available from: https://sid.ir/paper/317771/en

IEEE: Copy

Estela T. Mendez Olvera, Jaime A. Bustos Martinez, Yolanda Lopez Vidal, Antonio Verdugo Rodriguez, and Daniel Martinez Gomez, “Cytolethal Distending Toxin From Campylobacter jejuni Requires the Cytoskeleton for Toxic Activity,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317771/en

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