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Information Journal Paper

Title

Immunization with Protein D from Non-Typeable Haemophilus influenzae (NTHi) Induced Cytokine Responses and Bioactive Antibody Production

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Abstract

 Background: Outer membrane Protein D (PD) is a highly conserved and stable protein in the outer membrane of both encapsulated (typeable) and non-capsulated (non-typeable) strains of Haemophilus influenzae. As an immunogen, PD is a potential candidate vaccine against non-typeable H. influenzae (NTHi) strains. Objectives: The aim of this study was to determine the cytokine pattern and the opsonic antibody response in a BALB/c mouse model versus PD from NTHi as a vaccine candidate. Methods: Protein D was formulated with Freund’ s and outer membrane vesicle (OMV) adjuvants and injected into experimental mice. Sera from all groups were collected. The bioactivity of the anti-PD antibody was determined by opsonophagocytic killing test. To evaluate the cytokine responses, the spleens were assembled, suspension of splenocytes was recalled with antigen, and culture supernatants were analyzed by ELISA for IL-4, IL-10, and IFN- cytokines. Results: Anti-PD antibodies promoted phagocytosis of NTHi in both immunized mice groups (those administered PD + Freund’ s and those administered PD +OMVadjuvants, 92. 8% and 83. 5%, respectively, compared to the control group). In addition, the concentrations of three cytokines were increased markedly in immunized mice. Conclusions: We conclude that immunization with PD protects mice against NTHi. It is associated with improvements in both cellular and humoral immune responses and opsonic antibody activity.

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    APA: Copy

    Davoudi Vijeh Motlagh, Atefeh, SIADAT, SEYED DAVAR, ABEDIAN KENARI, SAEID, MAHDAVI, MEHDI, Behrouzi, Ava, & ASGARIAN OMRAN, HOSSEIN. (2016). Immunization with Protein D from Non-Typeable Haemophilus influenzae (NTHi) Induced Cytokine Responses and Bioactive Antibody Production. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(10), 0-0. SID. https://sid.ir/paper/317773/en

    Vancouver: Copy

    Davoudi Vijeh Motlagh Atefeh, SIADAT SEYED DAVAR, ABEDIAN KENARI SAEID, MAHDAVI MEHDI, Behrouzi Ava, ASGARIAN OMRAN HOSSEIN. Immunization with Protein D from Non-Typeable Haemophilus influenzae (NTHi) Induced Cytokine Responses and Bioactive Antibody Production. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(10):0-0. Available from: https://sid.ir/paper/317773/en

    IEEE: Copy

    Atefeh Davoudi Vijeh Motlagh, SEYED DAVAR SIADAT, SAEID ABEDIAN KENARI, MEHDI MAHDAVI, Ava Behrouzi, and HOSSEIN ASGARIAN OMRAN, “Immunization with Protein D from Non-Typeable Haemophilus influenzae (NTHi) Induced Cytokine Responses and Bioactive Antibody Production,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317773/en

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