The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing

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JOMEHPOUR NAJMEH; ESLAMI GILDA; KHALILI MOHAMMAD BAGHER

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:10 Page(s): 0-0

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Title

The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing

Author(s)

JOMEHPOUR NAJMEH | ESLAMI GILDA | KHALILI MOHAMMAD BAGHER | Issue Writer Certificate

Keywords

Methicillin-resistant Staphylococcus aureus

Methicillin-sensitive S.aureus

Ferula assa-foetida L

Carum copticum

Abstract

Background: Quorum sensing is a microbial cell-to-cell communication process. Quorum sensing bacteria produce and release extracellular messenger molecules called autoinducers. Gram-positive and Gram-negative, homoserine lactones, and oligopeptides are autoinducers used to communicate and regulate gene expression. Objectives: The goal of this study was to assess the impact of subinhibitory concentrations of Ferula assa-foetida L oleo-gum resin and Carum copticum fruit on the expression of tst and hld genes of Methicillin-resistant Staphylococcus aureus (MRSA) and methicillinsensitive S. aureus (MSSA) strains. Methods: This analytical study was performed using standard strains of MRSA (ATCC 33591) and MSSA (ATCC 29213). Suspensions of MRSA and MSSA bacteria were incubated at 37° C for 7 and 16 hours in the presence of ethanol extracts from F. assa-foetida and C. copticum. The expression of the hld and tst genes was then assessed using the real-time PCR protocol and SYBR Green Master Mix. The data analysis was carried out using the 2- CT method. Results: The hld gene expression (RNAIII) of MRSA after 7 and 16 hours of exposure to the sMIC of the F. assa-foetida extract showed a fold change of-1 and 0. 08, respectively, in comparison with controls. After 7 and 16 hours of exposure to the sMIC of the C. copticum extract, the fold change was-0. 23 and-0. 27, respectively. After exposure to the sMIC of the C. copticum extract for 16 hours, the fold change in the expression of the tst (TSST-1) MSSA gene was 0. 37 lower than that of the control sample. Conclusions: The results indicate that sMICs of ethanol extracts from F. assa-foetida and C. copticum can be used to control the expression of virulence genes in pathogenic bacteria, such as MRSA and MSSA.

Cites

Detection of Gene Encoding Enterotoxin A in Staphylococcus aureus Isolated from Cream Pastries

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APA: Copy

JOMEHPOUR, NAJMEH, ESLAMI, GILDA, & KHALILI, MOHAMMAD BAGHER. (2016). The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(10), 0-0. SID. https://sid.ir/paper/317778/en

Vancouver: Copy

JOMEHPOUR NAJMEH, ESLAMI GILDA, KHALILI MOHAMMAD BAGHER. The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(10):0-0. Available from: https://sid.ir/paper/317778/en

IEEE: Copy

NAJMEH JOMEHPOUR, GILDA ESLAMI, and MOHAMMAD BAGHER KHALILI, “The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317778/en

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