Reproducibility of 15-Loci MIRU-VNTR Method in Mycobacterium tuberculosis Genotyping

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Kochkaksaraei Maya Babai; KABOOSI HAMI; GHAEMI EZZAT ALLAH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2019 | Volume:12 | Issue:8 Page(s): 0-0

Download Full-Text

View:

192

Download:

138

Cites:

Information Journal Paper

Title

Reproducibility of 15-Loci MIRU-VNTR Method in Mycobacterium tuberculosis Genotyping

Author(s)

Kochkaksaraei Maya Babai | KABOOSI HAMI | GHAEMI EZZAT ALLAH | Issue Writer Certificate

Keywords

Mycobacterium tuberculosis

Minisatellite Repeats

Genotype

Reproducibility

Abstract

Background: Mycobacterium tuberculosis genotyping is an essential step for understanding the epidemiology of tuberculosis. Mycobacterial-interspersed-repetitive-units (MIRU)-variable-number-of-tandem-repeats (VNTR) typing is an important method for this purpose. Objectives: The study aimed to determine the Reproducibility of 15-loci MIRU-VNTR method. Methods: DNA extraction and 15-loci MIRU-VNTR were carried out for genotyping of 60 M. tuberculosis isolates collected from different clinical samples of 27 M. tuberculosis patients, each with two to four clinical isolates of M. tuberculosis. The similarity of M. tuberculosis isolates of the same patient was investigated by MIRU-VNTRplus. Results: The patterns of MIRU-VNTR were identical in 43 M. tuberculosis isolates collected from 20 tuberculosis patients, giving the repeatability of 71. 6% for the test. In 12 isolates of M. tuberculosis belonging to five patients, the variable-number tandem repeat (VNTR) in only one locus was different. In three M. tuberculosis isolates of one patient, two different Genotypes were identified. Conclusions: This study confirms the high Reproducibility of 15-loci MIRU-VNTR, except for limited cases.

Cites

No record.

References

No record.

Cite

APA: Copy

Kochkaksaraei, Maya Babai, KABOOSI, HAMI, & GHAEMI, EZZAT ALLAH. (2019). Reproducibility of 15-Loci MIRU-VNTR Method in Mycobacterium tuberculosis Genotyping. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 12(8), 0-0. SID. https://sid.ir/paper/317802/en

Vancouver: Copy

Kochkaksaraei Maya Babai, KABOOSI HAMI, GHAEMI EZZAT ALLAH. Reproducibility of 15-Loci MIRU-VNTR Method in Mycobacterium tuberculosis Genotyping. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2019;12(8):0-0. Available from: https://sid.ir/paper/317802/en

IEEE: Copy

Maya Babai Kochkaksaraei, HAMI KABOOSI, and EZZAT ALLAH GHAEMI, “Reproducibility of 15-Loci MIRU-VNTR Method in Mycobacterium tuberculosis Genotyping,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 12, no. 8, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/317802/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops