Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis

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Heidarnejadi Seyede Manizhe; RAFIEI ABDOLLAH; MAKVANDI MANOOCHEHR; PIRESTANI MAJID; GHADIRI ATAALLAH

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Journal Paper

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Journal: Jundishapur Journal of Microbiology Year:2017 | Volume:10 | Issue:7 Page(s): 00-00

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Title

Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis

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Keywords

Iowa Strain

Purification

Neonatal Rat

Cryptosporidium parvum

Abstract

Background: Cryptosporidiosis is a major public health problem for neonatal livestock worldwide. Cryptosporidium parvum infects intestinal epithelial cells via contaminated food or drinking water and leads to cryptosporidiosis. Most of the animal model studies on infectivity of C. parvumare conducted on the neonatal mice. Objectives: The current study aimed at evaluating the infectivity of C. parvum in Neonatal Rat as an animal model. Methods: A dose of 100, 000 to 120, 000 C. parvum oocysts (Iowa Strain, BTF Company, Sydney, Australia) was orally inoculated in a group of 30 neonatal Wistar rats aged 2 days old. Eight days postinfection, jejunum, ileum, cecum, colon, and rectum were removed and contents were homogenized and purified using sucrose gradient method. Results: Our results indicated that 6 to 12 million C. parvum was found per rat Conclusions: Analysis of the study results revealed that the Neonatal Rat could be used as an alternative animal model to investigate C. parvum.

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APA: Copy

Heidarnejadi, Seyede Manizhe, RAFIEI, ABDOLLAH, MAKVANDI, MANOOCHEHR, PIRESTANI, MAJID, & GHADIRI, ATAALLAH. (2017). Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 10(7), 00-00. SID. https://sid.ir/paper/317880/en

Vancouver: Copy

Heidarnejadi Seyede Manizhe, RAFIEI ABDOLLAH, MAKVANDI MANOOCHEHR, PIRESTANI MAJID, GHADIRI ATAALLAH. Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2017;10(7):00-00. Available from: https://sid.ir/paper/317880/en

IEEE: Copy

Seyede Manizhe Heidarnejadi, ABDOLLAH RAFIEI, MANOOCHEHR MAKVANDI, MAJID PIRESTANI, and ATAALLAH GHADIRI, “Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 10, no. 7, pp. 00–00, 2017, [Online]. Available: https://sid.ir/paper/317880/en

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