Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ROUDBARMOHAMMADI SHAHLA; SALIMI KHORASHAD ALIREZA; FOROUZANDEH MOGHADAM MEHDI; ROUDBARY MARYAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2018 | Volume:11 | Issue:2 Page(s): 0-0

Download Full-Text

View:

147

Download:

Cites:

Information Journal Paper

Title

Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique

Author(s)

ROUDBARMOHAMMADI SHAHLA | SALIMI KHORASHAD ALIREZA | FOROUZANDEH MOGHADAM MEHDI | ROUDBARY MARYAM | Issue Writer Certificate

Keywords

Not Registered.

Abstract

Background: Invasive aspergillosis (IA), a serious and fatal disease, is caused by numerous opportunistic fungi including Aspergillus species. Lack of early diagnosis and delay in treatment lead to the rapid spread of the infection and relapse, increase treatment costs, and ultimately cause death. Objectives: This study aimed at investigating the presence of Aspergillus species by Galactomannan EIA (GM) and TaqMan q PCR methods. Methods: Fluid samples of bronchoalveolar lavage (BAL) were collected from 89 patients, who were at risk for IA and underwent a bronchoscopy at Shariati hospital in Tehran, Iran. The specimens were examined using direct examination, culture methods, and GM and TaqMan real-time PCR. Results: A total of 23 samples were found to be positive in direct examination, 7 were identified as Aspergillus fumigatus, and 11 were positive as A. flavus in culture assays, 27 were GM positive, and 29 were positive with PAN Aspergillus probe. Moreover, 7 samples were positive with A. fumigatus probe and 11 were positive with A. flavus probe. Negative predictive value (NPV), sensitivity, positive predictive value (PPV), and specificity for a 1. 0 BAL GM level were 98. 4%, 94. 4%, 62. 9%, and 85. 9%, respectively, and were 100% for TaqMan Real time PCR. Conclusions: Our findings revealed that TaqManreal-time PCR has a great value for detection of Aspergillus species in BAL specimen. Since early diagnosis has been highly considered to prevent the occurrence of drug resistance and mortality rate, it is necessary to use this method directly to detect Aspergillus species in BAL or blood specimens without culture. Onthe other hand, Galactomannan assay with a 98. 4% NPVcould be usedto screen the patients with suspected invasive aspergillosis. Additionally, dueto the lack of need for specialized devices, an appropriate diagnostic technique should be used in laboratories that do not have access to specializGalactomannan,Real Time PCR,Clinical Samples,Bronchoalveolar Lavage,Aspergillus Detectioned procedures.

Cites

No record.

References

No record.

Cite

APA: Copy

ROUDBARMOHAMMADI, SHAHLA, SALIMI KHORASHAD, ALIREZA, FOROUZANDEH MOGHADAM, MEHDI, & ROUDBARY, MARYAM. (2018). Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 11(2), 0-0. SID. https://sid.ir/paper/317944/en

Vancouver: Copy

ROUDBARMOHAMMADI SHAHLA, SALIMI KHORASHAD ALIREZA, FOROUZANDEH MOGHADAM MEHDI, ROUDBARY MARYAM. Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2018;11(2):0-0. Available from: https://sid.ir/paper/317944/en

IEEE: Copy

SHAHLA ROUDBARMOHAMMADI, ALIREZA SALIMI KHORASHAD, MEHDI FOROUZANDEH MOGHADAM, and MARYAM ROUDBARY, “Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan Enzyme Assay and TaqMan Real-Time PCR Technique,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 11, no. 2, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/317944/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops