The Effect of Placenta Growth FactorKnockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, andhsa-mir-4290 Expressions in MKN-45-derived Gastric Cancer Stem-like Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Salehi Zohreh; AKRAMI HASSAN; SISAKHTNEZHAD SAJJAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MIDDLE EAST JOURNAL OF CANCER Year:2018 | Volume:9 | Issue:2 Page(s): 113-122

Download Full-Text

View:

241

Download:

130

Cites:

Information Journal Paper

Title

The Effect of Placenta Growth FactorKnockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, andhsa-mir-4290 Expressions in MKN-45-derived Gastric Cancer Stem-like Cells

Author(s)

Salehi Zohreh | AKRAMI HASSAN | SISAKHTNEZHAD SAJJAD | Issue Writer Certificate

Keywords

PlGF

Cancer stem cell

miRNA

MKN-45

Abstract

Background: Placental growth factor is involved in human gastric cancer initiationand progression through stimulating the proliferation, angiogenesis, invasion andmetastasis of cancerous cells. Previous studies indicate that the expression profiles ofhsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, and hsa-mir-4290 change in MKN-45-derived gastric cancer stem-like cells. Therefore, this study aims to investigate the effectof PlGF knockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, and hsa-mir-4290expressions in MKN-45-derived gastric cancer stem-like cells. Methods: We used a non-adhesive culture system to derive the cancer stem-likecells from MKN-45 cells. PlGF gene silencing was performed by PlGF-specificsiRNA. The transcript of PlGF and miRNAs were measured by real-time RT-PCR. Weconducted bioinformatics analyses with the online software programs TargetScan, miRanda, miRWalk, PicTar, and the Database for Annotation, Visualization, andIntegrated Discovery tools to predict miRNAs’ targets and their signaling pathways. Results: hsa-let-7b-3p had a 2. 28-fold up-regulation, whereas we observed downregulationof hsa-mir-451b (25%), hsa-mir-4290 (34%), and hsa-mir-22-3p (9%). Bioinformatics analysis results indicated that the miRNA target genes TGF-β , MAPK, and Wnt, and hedgehog signaling pathways contributed to cancer initiation andprogression by influencing different cellular behaviors. Conclusion: We suggest that PlGF signaling may influence miRNA expressionprofiles in MKN-45-derived cancer stem-like cells, which can influence the expressionsof different genes and signaling pathways. However, more empirical studies shoulddetermine the exact effect of PlGF knockdown on the expression of miRNA targetsin cancer stem-like cells to locate their actual gene targets.

Cites

No record.

References

No record.

Cite

APA: Copy

Salehi, Zohreh, AKRAMI, HASSAN, & SISAKHTNEZHAD, SAJJAD. (2018). The Effect of Placenta Growth FactorKnockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, andhsa-mir-4290 Expressions in MKN-45-derived Gastric Cancer Stem-like Cells. MIDDLE EAST JOURNAL OF CANCER, 9(2), 113-122. SID. https://sid.ir/paper/321232/en

Vancouver: Copy

Salehi Zohreh, AKRAMI HASSAN, SISAKHTNEZHAD SAJJAD. The Effect of Placenta Growth FactorKnockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, andhsa-mir-4290 Expressions in MKN-45-derived Gastric Cancer Stem-like Cells. MIDDLE EAST JOURNAL OF CANCER[Internet]. 2018;9(2):113-122. Available from: https://sid.ir/paper/321232/en

IEEE: Copy

Zohreh Salehi, HASSAN AKRAMI, and SAJJAD SISAKHTNEZHAD, “The Effect of Placenta Growth FactorKnockdown on hsa-miR-22-3p, hsa-let-7b-3p, hsa-miR-451b, andhsa-mir-4290 Expressions in MKN-45-derived Gastric Cancer Stem-like Cells,” MIDDLE EAST JOURNAL OF CANCER, vol. 9, no. 2, pp. 113–122, 2018, [Online]. Available: https://sid.ir/paper/321232/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops