The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line

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ROSHANKHAH SHIVA; Arji Rodsari Babak; JALILI CYRUS; SALAHSHOOR MOHAMMAD REZA

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Journal: MIDDLE EAST JOURNAL OF CANCER Year:2020 | Volume:11 | Issue:1 Page(s): 34-41

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Title

The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line

Author(s)

ROSHANKHAH SHIVA | Arji Rodsari Babak | JALILI CYRUS | SALAHSHOOR MOHAMMAD REZA | Issue Writer Certificate

Keywords

Harmine

P53 gene

MCF-7

Apoptosis

Cell line

Abstract

Background: As a major tumor suppressor gene, P53 plays a principal role in the Apoptosis of cancer cells. Harmine is a harmal-derived alkaloid with antioxidant and anticancer properties. This study was designed to assess the ability of Harmine to express P53 gene and stimulate Apoptosis in breast cancer Cell line. Methods: MCF-7 Cell line was cultured and treated with Harmine. Half maximal inhibitory concentration (IC50) assay was carried out through MMT method following 24 h of treatment. Flow cytometry technique was employed to measure apoptotic cells and real-time PCR was performed to estimate P53 gene expression in tumor cells. Results: The IC50 for the Harmine extract in MCF-7 cells was 30 μ M. Harmine administration in all treated groups resulted in a significant increase in Apoptosis and P53 gene expression in MCF-7 cells (P < 0. 00001). Conclusions: It seems that Harmine administration is able to induce Apoptosis in MCF-7 cells through the up-regulation of P53 expression.

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APA: Copy

ROSHANKHAH, SHIVA, Arji Rodsari, Babak, JALILI, CYRUS, & SALAHSHOOR, MOHAMMAD REZA. (2020). The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line. MIDDLE EAST JOURNAL OF CANCER, 11(1), 34-41. SID. https://sid.ir/paper/321319/en

Vancouver: Copy

ROSHANKHAH SHIVA, Arji Rodsari Babak, JALILI CYRUS, SALAHSHOOR MOHAMMAD REZA. The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line. MIDDLE EAST JOURNAL OF CANCER[Internet]. 2020;11(1):34-41. Available from: https://sid.ir/paper/321319/en

IEEE: Copy

SHIVA ROSHANKHAH, Babak Arji Rodsari, CYRUS JALILI, and MOHAMMAD REZA SALAHSHOOR, “The Role of Harmine in Up-regulating p53 Gene Expression and Inducing Apoptosis in MCF-7 Cell Line,” MIDDLE EAST JOURNAL OF CANCER, vol. 11, no. 1, pp. 34–41, 2020, [Online]. Available: https://sid.ir/paper/321319/en

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