MODIFICATION OF GLUCOSE BIOSENSOR USING PT/MWCNTS ELECTRODE AND OPTIMIZATION BY APPLICATION OF TAGUCHI METHOD

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RAMEZANI AZGHANDI OMID; MAGHREBI MOHAMMAD JAVAD; TEYMOURTASH ALIREZA

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Journal Paper

Paper Information

Journal: International Journal of Nano Dimension Year:2016 | Volume:7 | Issue:3 Page(s): 231-239

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Information Journal Paper

Title

MODIFICATION OF GLUCOSE BIOSENSOR USING PT/MWCNTS ELECTRODE AND OPTIMIZATION BY APPLICATION OF TAGUCHI METHOD

Author(s)

RAMEZANI AZGHANDI OMID | MAGHREBI MOHAMMAD JAVAD | TEYMOURTASH ALIREZA | Issue Writer Certificate

Keywords

CARBON NANOTUBES

ENZYME GLUCOSE OXIDASE

GOLD NANOPARTICLES

PLATINUM ELECTRODE

SOFTWARE QUALTEK-4

Abstract

In this paper, multi-wall CARBON NANOTUBES (MWCNTs), GOLD NANOPARTICLES (GNp) and glucose oxidase (GOD) was developed for the specific detection of glucose. MWCNTs were chemically modified with the H2SO4–HNO3 pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (PAA) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide cross-linking reaction, respectively. A cleaned Pt electrode was immersed in PAA, MWCNTs, cysteamine and GNp, respectively, followed by the adsorption of GOD, assembling the one layer of films on the surface of Pt electrode (GOD/GNp/MWCNTs/Pt electrode) and was used as working electrodes (anode) along with a platinum auxiliary electrode and the reference electrode Ag/AgCl (cathode). Working electrode was containing the Phosphate-buffered saline (PBS) with pH = 4, 6, 8 enzyme. To evaluate the performance of the constructed biosensor, it was put into a dish containing 0.05, 0.5 and 1 mmol lit-1 (mM) glucose concentrations which were dissolved in doubly distilled water. The produced current and its rate were measured by Potentiostat with transferring the electron from the working electrode to reference electrode which was released by the reaction of glucose in the presence of the enzyme. Glucose concentration and PBS pH design has been tested and analyzed by QUALTEK-4 software measure. According to the performed experiments and software analysis, with increasing concentration, the flow rate of current production is increased and pH deviance from neutral range reduces the flow. Optimal conditions was obtained in concentrations 1 mmol lit-1 and pH =6, respectively. After confirmation tests in optimum conditions, the rate of production was obtained, 21.67 mA, which with respect to the expected error rate of application, it was calculated to be 8.1%. This error rate demonstrates that the accuracy of tests is with high sensitivity and accuracy.

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References

THE OBTAIN OPTIMUM PRODUCTION CONDITIONS FOR GLUCOSE OXIDASE BIOSENSOR USING SOFTWARE QUALTEK-4

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APA: Copy

RAMEZANI AZGHANDI, OMID, MAGHREBI, MOHAMMAD JAVAD, & TEYMOURTASH, ALIREZA. (2016). MODIFICATION OF GLUCOSE BIOSENSOR USING PT/MWCNTS ELECTRODE AND OPTIMIZATION BY APPLICATION OF TAGUCHI METHOD. INTERNATIONAL JOURNAL OF NANO DIMENSION (IJND), 7(3), 231-239. SID. https://sid.ir/paper/322316/en

Vancouver: Copy

RAMEZANI AZGHANDI OMID, MAGHREBI MOHAMMAD JAVAD, TEYMOURTASH ALIREZA. MODIFICATION OF GLUCOSE BIOSENSOR USING PT/MWCNTS ELECTRODE AND OPTIMIZATION BY APPLICATION OF TAGUCHI METHOD. INTERNATIONAL JOURNAL OF NANO DIMENSION (IJND)[Internet]. 2016;7(3):231-239. Available from: https://sid.ir/paper/322316/en

IEEE: Copy

OMID RAMEZANI AZGHANDI, MOHAMMAD JAVAD MAGHREBI, and ALIREZA TEYMOURTASH, “MODIFICATION OF GLUCOSE BIOSENSOR USING PT/MWCNTS ELECTRODE AND OPTIMIZATION BY APPLICATION OF TAGUCHI METHOD,” INTERNATIONAL JOURNAL OF NANO DIMENSION (IJND), vol. 7, no. 3, pp. 231–239, 2016, [Online]. Available: https://sid.ir/paper/322316/en

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