Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

278
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

133
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma

Pages

  75-82

Abstract

 Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. k-ras Codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k-ras gene mutations and CA125 tumor marker in patients with Ovarian carcinoma in Tabriz city. Methods: Genomic DNA was extracted from 67 tissues pertained to women with Ovarian carcinoma. Mutations in Codon 12 and 13 of k-ras gene were analyzed by Nested PCR and RFLP methods. Titer of CA125 tumor marker was determined by ELISA. Results: Among the 67 patients, 7 patients (10. 4%) had mutation in k-ras Codon 12 while none of them had mutation in k-ras codon 13. Based on the results, the frequency of various genotypes were 89. 55%, 10. 44%, and 0%, for GG, GA, and AA alleles, respectively. The p-value for stage I and Grade I tumors were 0. 022 and 0. 04, respectively, indicating a statistically significant correlation between Codon 12 mutation and stage I and Grade I tumors. Furthermore, we found significant correlations among CA125 tumor marker titers and histological grade (p<0. 000) and stage (p<0. 000). The mean serum CA125 tumor marker levels in malignant carcinomas were 499. 84 U/ml. Conclusion: The results in this study indicated high prevalence of k-ras Codon 12 mutations and high titer of CA125 tumor marker in patients with Ovarian carcinoma in the study region.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Bagherlou, Nazanin, FARAJNIA, SAFAR, ZAHRI, SABER, DASTRANJ TABRIZI, ALI, & Nazari, Atefeh. (2020). Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma. CASPIAN JOURNAL OF INTERNAL MEDICINE, 11(1), 75-82. SID. https://sid.ir/paper/323590/en

    Vancouver: Copy

    Bagherlou Nazanin, FARAJNIA SAFAR, ZAHRI SABER, DASTRANJ TABRIZI ALI, Nazari Atefeh. Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma. CASPIAN JOURNAL OF INTERNAL MEDICINE[Internet]. 2020;11(1):75-82. Available from: https://sid.ir/paper/323590/en

    IEEE: Copy

    Nazanin Bagherlou, SAFAR FARAJNIA, SABER ZAHRI, ALI DASTRANJ TABRIZI, and Atefeh Nazari, “Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma,” CASPIAN JOURNAL OF INTERNAL MEDICINE, vol. 11, no. 1, pp. 75–82, 2020, [Online]. Available: https://sid.ir/paper/323590/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top