CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS

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HARRIS JAMIE; KAJDACSY BALLA ANDRE; CHIU BILL

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Journal Paper

Paper Information

Journal: Gastroenterology and Hepatology From Bed to Bench Year:2016 | Volume:9 | Issue:3 Page(s): 174-179

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Title

CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS

Author(s)

HARRIS JAMIE | KAJDACSY BALLA ANDRE | CHIU BILL | Issue Writer Certificate

Keywords

HEPATOMA

ULTRASOUND

METASTATIC

MOUSE

ORTHOTOPIC

Abstract

Aim: To create an ORTHOTOPIC HEPATOMA model with local metastasis monitored with ULTRASOUND could be created as a platform for testing new treatments.Background: HEPATOMA accounts for 25% of liver tumors in children with poor overall survival. Intraabdominal metastasis are present in 35% of patients at time of diagnosis. We hypothesized that an ORTHOTOPIC tumor model with local metastasis could be created as a platform for testing treatment modalities and could be monitored with ULTRASOUND.Patients and methods: One million human HEPATOMA cells (Hep3B) were injected into the left lobe of the liver of immunocompromised mice. Tumor volume was monitored with high frequency-ultrasound until it reached 1,000mm3. At that time animals were sacrificed and examined for gross METASTATIC disease. Tumor sections were analyzed with hematoxylin and eosin (H&E) staining.Results: Tumor formed in 8.15 mice. The tumor was detected as small as 19.59mm3 on ULTRASOUND. Of the forming tumors, tumor size was 145±177.93mm3 at 60 days post-injection, 665±650.39mm3 at 67 days, and reached >1000mm3 by 76.6±9.9 days. At necropsy, four mice (50%) had tumor only within the liver, four (50%) had additional tumors in omentum, pelvis and peritoneum. H&E showed tumor within the normal liver parenchyma, with multiple mitotic figures, small areas of necrosis, and hemorrhage within the tumor.Conclusion: We have successfully established an ORTHOTOPIC HEPATOMA murine model, with a local METASTATIC rate of 50%. Non-invasive tumor monitoring is feasible via ULTRASOUND.

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APA: Copy

HARRIS, JAMIE, KAJDACSY BALLA, ANDRE, & CHIU, BILL. (2016). CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, 9(3), 174-179. SID. https://sid.ir/paper/324657/en

Vancouver: Copy

HARRIS JAMIE, KAJDACSY BALLA ANDRE, CHIU BILL. CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH[Internet]. 2016;9(3):174-179. Available from: https://sid.ir/paper/324657/en

IEEE: Copy

JAMIE HARRIS, ANDRE KAJDACSY BALLA, and BILL CHIU, “CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS,” GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, vol. 9, no. 3, pp. 174–179, 2016, [Online]. Available: https://sid.ir/paper/324657/en

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