Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR

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SHARIFDINI MEYSAM; KEYHANI AMIR; ESHRAGHIAN MOHAMMAD REZA; BEIGOM KIA ESHRAT

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Paper Information

Journal: Gastroenterology and Hepatology From Bed to Bench Year:2018 | Volume:11 | Issue:1 Page(s): 68-74

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Title

Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR

Author(s)

SHARIFDINI MEYSAM | KEYHANI AMIR | ESHRAGHIAN MOHAMMAD REZA | BEIGOM KIA ESHRAT | Issue Writer Certificate

Keywords

elderly

molecular diagnosis

nested-PCR

Strongyloides stercoralis

Abstract

Aim: This study is aimed to diagnose and analyze strongyloidiasis in a population of an endemic area of Iran using nested-PCR, coupled with parasitological methods. Background: Screening of strongyloidiasis infected people using reliable diagnostic techniques are essential to decrease the mortality and morbidity associated with this infection. Molecular methods have been proved to be highly sensitive and specific for detection of Strongyloides stercoralis in stool samples. Methods: A total of 155 fresh single stool samples were randomly collected from residents of north and northwest of Khouzestan Province, Iran. All samples were examined by parasitological methods including formalin-ether concentration and nutrient agar plate culture, and molecular method of nested-PCR. Infections with S. stercoralis were analyzed according to demographic criteria. Results: Based on the results of nested-PCR method 15 cases (9. 7%) were strongyloidiasis positive. nested-PCR was more sensitive than parasitological techniques on single stool sampling. elderly was the most important population index for higher infectivity with S. stercoralis. Conclusion: In endemic areas of S. stercoralis, old age should be considered as one of the most important risk factors of infection, especially among the immunosuppressed individuals.

Cites

Parasitological, Immunological, and Molecular Methods in Diagnosis of Human Strongyloidiasis (Review Paper)

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APA: Copy

SHARIFDINI, MEYSAM, KEYHANI, AMIR, ESHRAGHIAN, MOHAMMAD REZA, & BEIGOM KIA, ESHRAT. (2018). Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, 11(1), 68-74. SID. https://sid.ir/paper/324752/en

Vancouver: Copy

SHARIFDINI MEYSAM, KEYHANI AMIR, ESHRAGHIAN MOHAMMAD REZA, BEIGOM KIA ESHRAT. Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH[Internet]. 2018;11(1):68-74. Available from: https://sid.ir/paper/324752/en

IEEE: Copy

MEYSAM SHARIFDINI, AMIR KEYHANI, MOHAMMAD REZA ESHRAGHIAN, and ESHRAT BEIGOM KIA, “Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR,” GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, vol. 11, no. 1, pp. 68–74, 2018, [Online]. Available: https://sid.ir/paper/324752/en

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