COMPARISON OF ER:YAG LASER AND HAND INSTRUMENTATION ON THE ATTACHMENT OF CULTURED HUMAN GINGIVAL FIBROBLASTS TO PERIODONTALLY INVOLVED ROOT SURFACES

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TALEBI ARDAKANI MOHAMMAD REZA; TORSHABI MARYAM; KARAMI ELAHE; VAJAR NAZANIN; REZAEI ESFAHROOD ZEINAB; MEIMANDI MANSOUR; MOJAHEDI SEYED MASOUD

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Journal: JOURNAL OF LASERS IN MEDICAL SCIENCES Year:2017 | Volume:8 | Issue:SUPPLEMENT 1 Page(s): 51-55

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Title

COMPARISON OF ER:YAG LASER AND HAND INSTRUMENTATION ON THE ATTACHMENT OF CULTURED HUMAN GINGIVAL FIBROBLASTS TO PERIODONTALLY INVOLVED ROOT SURFACES

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Keywords

SCALING

ROOT PLANNING

ER: YAG LASER

FIBROBLAST

VIABILITY

ATTACHMENT

Abstract

Introduction: The present study compared the effects of erbium-doped yttrium aluminium garnet (Er: YAG) laser and hand instrumentation on the ATTACHMENT of human gingival FIBROBLAST (HGF) cells to periodontally involved root surfaces.Methods: A total of 40 tooth specimens were collected and treated in four distinct groups: scaled and root planed with hand instruments, scaled with ER: YAG LASER, treated with a combination of hand instruments and ER: YAG LASER and non-treated control group. The ATTACHMENT and proliferation rate of HGF were assessed using MTT assay and scanning electron microscope (SEM) examination was used for cell morphological evaluation.Results: The MTT (3- [4, 5-dimethylthiazol-2-yl] -2, 5 diphenyl tetrazolium bromide) assay showed significant decrease in HGF cell VIABILITY in both hand instruments only and combination treated teeth specimens compared to control specimens (P<0.05), 24 hours after cell seeding. However, at time 48, the cell VIABILITY of attached cells in these 2 treated groups was almost similar to control. In contrast, at 24 and 48 hours after cell seeding, VIABILITY of attached cells was higher than control in ER: YAG LASER treated only specimens (P<0.05). According to SEM study, the laser treated specimens showed more surface roughness.Conclusion: ER: YAG LASER increased ATTACHMENT and proliferation of HGF cells in comparison to the hand instruments method.

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APA: Copy

TALEBI ARDAKANI, MOHAMMAD REZA, TORSHABI, MARYAM, KARAMI, ELAHE, VAJAR, NAZANIN, REZAEI ESFAHROOD, ZEINAB, MEIMANDI, MANSOUR, & MOJAHEDI, SEYED MASOUD. (2017). COMPARISON OF ER:YAG LASER AND HAND INSTRUMENTATION ON THE ATTACHMENT OF CULTURED HUMAN GINGIVAL FIBROBLASTS TO PERIODONTALLY INVOLVED ROOT SURFACES. JOURNAL OF LASERS IN MEDICAL SCIENCES, 8(SUPPLEMENT 1), 51-55. SID. https://sid.ir/paper/325925/en

Vancouver: Copy

TALEBI ARDAKANI MOHAMMAD REZA, TORSHABI MARYAM, KARAMI ELAHE, VAJAR NAZANIN, REZAEI ESFAHROOD ZEINAB, MEIMANDI MANSOUR, MOJAHEDI SEYED MASOUD. COMPARISON OF ER:YAG LASER AND HAND INSTRUMENTATION ON THE ATTACHMENT OF CULTURED HUMAN GINGIVAL FIBROBLASTS TO PERIODONTALLY INVOLVED ROOT SURFACES. JOURNAL OF LASERS IN MEDICAL SCIENCES[Internet]. 2017;8(SUPPLEMENT 1):51-55. Available from: https://sid.ir/paper/325925/en

IEEE: Copy

MOHAMMAD REZA TALEBI ARDAKANI, MARYAM TORSHABI, ELAHE KARAMI, NAZANIN VAJAR, ZEINAB REZAEI ESFAHROOD, MANSOUR MEIMANDI, and SEYED MASOUD MOJAHEDI, “COMPARISON OF ER:YAG LASER AND HAND INSTRUMENTATION ON THE ATTACHMENT OF CULTURED HUMAN GINGIVAL FIBROBLASTS TO PERIODONTALLY INVOLVED ROOT SURFACES,” JOURNAL OF LASERS IN MEDICAL SCIENCES, vol. 8, no. SUPPLEMENT 1, pp. 51–55, 2017, [Online]. Available: https://sid.ir/paper/325925/en

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