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Information Journal Paper

Title

Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients

Pages

  416-431

Keywords

Syzygium aromaticum (L.) 

Abstract

 Objective: Considering the emergence of biofilm-associated pathogens with multidrug resistance, the objective(s) of the present study was to evaluate the anti-virulence property of Syzygium aromaticum extracts/fractions against 2 multidrug-resistant catheter isolates. Materials and Methods: Pulverized clove buds were subjected to bioactivity-guided isolation to identify the bioactive extract/fraction(s) with significant anti-virulence property. The clove bud powder was subjected to Soxhlet extraction and all these extracts were investigated in terms of anti-virulent efficacy using initial readout assays. Bioassay-guided partial purification was performed through column and size exclusion chromatography. Detailed testing of the anti-virulence and anti-biofilm activity of sub-minimum inhibitory concentration (sub-MIC) levels of the active fraction, was performed besides the cytotoxicity profiling in RAW 264. 7 cells. Results: Gas chromatography-mass spectrometry (GC-MS) analysis of the clove bioactive fraction-2 (CBF-2), done after the bioassay-guided fractionation, revealed Eugenol as the major bioactive ingredient present in CBF-2. Reduced mRNA levels of virulence factor genes after CBF-2 (700 μ g/ml) treatment correlated well with the respective phenotypic assays (p<0. 001). Similarly, CBF-2 (700 μ g/ml) treatment exhibited significantly low mRNA levels of Quorum sensing (QS) receptor genes compared to their cognate synthase genes (p<0. 001). Crystal violet staining and scanning electron micrographs of CBF-2-treated biofilms showed lesser macrocolonies with remarkably simplified architecture. Cytotoxic evaluation of CBF-2 suggested a minute reduction in viability even at the highest tested concentration (1600 μ g/ml, p<0. 05). Conclusion: The present study indicated that Eugenol-rich CBF-2 has potent anti-virulence and anti-biofilm activity against urinary catheter isolates and can be regarded as an alternative for treatment of catheter-associated urinary tract infections.

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    APA: Copy

    Rathinam, Prasanth, & VISWANATHAN, PRAGASAM. (2018). Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 8(5), 416-431. SID. https://sid.ir/paper/326422/en

    Vancouver: Copy

    Rathinam Prasanth, VISWANATHAN PRAGASAM. Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2018;8(5):416-431. Available from: https://sid.ir/paper/326422/en

    IEEE: Copy

    Prasanth Rathinam, and PRAGASAM VISWANATHAN, “Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 8, no. 5, pp. 416–431, 2018, [Online]. Available: https://sid.ir/paper/326422/en

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