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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF EUROTIUM SPECIES FROM CONTAMINATED RICE BY MORPHOLOGY AND DNA SEQUENCING

Pages

  97-102

Abstract

 30 milled RICE samples were collected from retailers in four states of Malaysia. These samples were evaluated for EUROTIUM spp. contaminations by direct plating on malt extract salt agar (MESA). All EUROTIUM were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA. Four EUROTIUM species (E. rubrum, E. amstelodami, E. chevalieri and E. cristatum) dominated seed samples were identified. The main characteristics for morphological differentiation of EUROTIUM species were colony features on different culture media and ascospore surface ornamentations. The PCR-sequencing technique for sequences of ITS1 and ITS2 is a fast technique for identification of EUROTIUM species, but did not work perfectly for differentiating EUROTIUM species from each others. DNA sequence analysis showed a fixed sequence numbers in both ITS1 and ITS2 regions. These results suggest that sequencing of ITS regions could support MORPHOLOGICAL CHARACTERISTICS for identification of EUROTIUM species.

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  • Cite

    APA: Copy

    YAZDANI, DARAB, MIOR AHMAD, ZAINAL ABIDIN, HOW, TAN YEE, & QADERI, ARDESHIR. (2011). ISOLATION AND IDENTIFICATION OF EUROTIUM SPECIES FROM CONTAMINATED RICE BY MORPHOLOGY AND DNA SEQUENCING. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 1(2), 97-102. SID. https://sid.ir/paper/328046/en

    Vancouver: Copy

    YAZDANI DARAB, MIOR AHMAD ZAINAL ABIDIN, HOW TAN YEE, QADERI ARDESHIR. ISOLATION AND IDENTIFICATION OF EUROTIUM SPECIES FROM CONTAMINATED RICE BY MORPHOLOGY AND DNA SEQUENCING. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2011;1(2):97-102. Available from: https://sid.ir/paper/328046/en

    IEEE: Copy

    DARAB YAZDANI, ZAINAL ABIDIN MIOR AHMAD, TAN YEE HOW, and ARDESHIR QADERI, “ISOLATION AND IDENTIFICATION OF EUROTIUM SPECIES FROM CONTAMINATED RICE BY MORPHOLOGY AND DNA SEQUENCING,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 1, no. 2, pp. 97–102, 2011, [Online]. Available: https://sid.ir/paper/328046/en

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