DETECTION OF ISONIAZID-RESISTANT CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM INDIA USING SER315THR MARKER BY COMPARISON OF MOLECULAR METHODS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

A. NUSRATH UNISSA; NARAYANAN SUJATHA; SUGANTHI C.; SELVAKUMAR N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY Year:2011 | Volume:1 | Issue:2 Page(s): 52-59

Download Full-Text

View:

322

Download:

152

Cites:

Information Journal Paper

Title

DETECTION OF ISONIAZID-RESISTANT CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM INDIA USING SER315THR MARKER BY COMPARISON OF MOLECULAR METHODS

Author(s)

A. NUSRATH UNISSA | NARAYANAN SUJATHA | SUGANTHI C. | SELVAKUMAR N. | Issue Writer Certificate

Keywords

MYCOBACTERIUM TUBERCULOSIS

ISONIAZID RESISTANCE

DNA SEQUENCING

PCR-SSCP

PCR-RFLP

Abstract

In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA SEQUENCING, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of India. Out of the 105 resistant isolates, 64 (61%) were found to be resistant by DNA SEQUENCING, 54 (51%) by PCR-RFLP and 57 (54%) by PCR-SSCP methods. The results obtained using PCR-SSCP and PCR-RFLP methods were compared with those from DNA SEQUENCING (gold standard). The sensitivity and specificity of PCR-RFLP were 84% and 100% respectively and corresponding values for PCR-SSCP method were 89% and 95% respectively. The study has shown the comparison of the simple, rapid and cost effective methods with DNA SEQUENCING targeting codon Ser315 of katG gene and suggests that PCR-RFLP and PCRSSCP may be performed as alternative inexpensive methods in settings with a high prevalence of INH-resistant M. tuberculosis strains where sequencing cannot be afforded.

Cites

No record.

References

No record.

Cite

APA: Copy

A. NUSRATH, UNISSA, NARAYANAN, SUJATHA, SUGANTHI, C., & SELVAKUMAR, N.. (2011). DETECTION OF ISONIAZID-RESISTANT CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM INDIA USING SER315THR MARKER BY COMPARISON OF MOLECULAR METHODS. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 1(2), 52-59. SID. https://sid.ir/paper/328049/en

Vancouver: Copy

A. NUSRATH UNISSA, NARAYANAN SUJATHA, SUGANTHI C., SELVAKUMAR N.. DETECTION OF ISONIAZID-RESISTANT CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM INDIA USING SER315THR MARKER BY COMPARISON OF MOLECULAR METHODS. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2011;1(2):52-59. Available from: https://sid.ir/paper/328049/en

IEEE: Copy

UNISSA A. NUSRATH, SUJATHA NARAYANAN, C. SUGANTHI, and N. SELVAKUMAR, “DETECTION OF ISONIAZID-RESISTANT CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM INDIA USING SER315THR MARKER BY COMPARISON OF MOLECULAR METHODS,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 1, no. 2, pp. 52–59, 2011, [Online]. Available: https://sid.ir/paper/328049/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops