PCR–RFLP OF ISOLATED SALMONELLA FROM POULTRY WITH SAU3AI AND HHAI RESTRICTION ENDONUCLEASES IN ARAK

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KHAKI PEJVAK; MORADI BIDHENDI SOHEILA; EZATPANAH ELHAM

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Journal: INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY Year:2013 | Volume:3 | Issue:1 Page(s): 255-260

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Title

PCR–RFLP OF ISOLATED SALMONELLA FROM POULTRY WITH SAU3AI AND HHAI RESTRICTION ENDONUCLEASES IN ARAK

Author(s)

KHAKI PEJVAK | MORADI BIDHENDI SOHEILA | EZATPANAH ELHAM | Issue Writer Certificate

Keywords

SALMONELLA

AVIAN

FLIC GENE

PCR-RFLP

HHAI

SAU3AI

ARAK

Abstract

SALMONELLA entericais considered as one of the major pathogens in public health, worldwide. Diseases caused by SALMONELLA enterica serovars are especially prevalent in developing countries. It is one of the emerging pathogen in food-borne diseases, which is often found in contaminated chicken eggs. The aim of this study was to apply a PCR–RFLP method using SAU3AI and HHAI restriction endonucleases on the FLIC GENE to identify the serotypes ofSALMONELLA isolates in AVIAN living in ARAK area in central Iran. Serotypes of 75 isolates included SALMONELLA enteritidis (45.33%), SALMONELLA infantis (44%), SALMONELLA typhimurium (5.33%), SALMONELLA bardo (2.67%) and SALMONELLA bacongo (2.67%). All the isolates showed FLIC GENE (1500bp) by using specific primers. The results of PCR-RFLP with restriction enzymes HHAI and SAU3AI for gene fliC showed 4 and 5 patterns. It was observed that SAU3AI is able to discriminate all serotypes of SALMONELLA but HHAI could not distinguish SALMONELLA infantis and SALMONELLA typhimurium. The prevalent SALMONELLA isolated in this study belonged to serotype enteritidis and infantis. The combination of the data obtained with both restriction enzymes could differentiate the serovars completely. These results showed that FLIC GENE is a suitable target gene for discriminating among these SALMONELLA serotypes by PCR-RFLP.

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APA: Copy

KHAKI, PEJVAK, MORADI BIDHENDI, SOHEILA, & EZATPANAH, ELHAM. (2013). PCR–RFLP OF ISOLATED SALMONELLA FROM POULTRY WITH SAU3AI AND HHAI RESTRICTION ENDONUCLEASES IN ARAK. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 3(1), 255-260. SID. https://sid.ir/paper/328081/en

Vancouver: Copy

KHAKI PEJVAK, MORADI BIDHENDI SOHEILA, EZATPANAH ELHAM. PCR–RFLP OF ISOLATED SALMONELLA FROM POULTRY WITH SAU3AI AND HHAI RESTRICTION ENDONUCLEASES IN ARAK. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2013;3(1):255-260. Available from: https://sid.ir/paper/328081/en

IEEE: Copy

PEJVAK KHAKI, SOHEILA MORADI BIDHENDI, and ELHAM EZATPANAH, “PCR–RFLP OF ISOLATED SALMONELLA FROM POULTRY WITH SAU3AI AND HHAI RESTRICTION ENDONUCLEASES IN ARAK,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 3, no. 1, pp. 255–260, 2013, [Online]. Available: https://sid.ir/paper/328081/en

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