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Cites:

1

Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUND PRODUCING RHODOCOCCUS SPP. ISOLATED FROM SOIL SAMPLES

Pages

  463-468

Abstract

 The genus Rhodococcus is gram positive bacterium which was classified into the family of Nocardiaceae. The organism can be isolates from different sources, with capability to degrade diverse types of pollutant. Nowadays, scientists reported a few secondary metabolites which are produced by RHODOCOCCI. Hence, the present study tried to isolate RHODOCOCCI from different SOIL SAMPLEs with the capability to produce antimicrobial metabolites. For this purpose 360 SOIL SAMPLEs were collected from different types of soil. Then the isolates were identified and characterized using APi Coryne kit. Afterward antibacterial activity of the suspected bacteria was evaluated using well diffusion agar method against test bacteria. Furthermore, the extracted metabolites were analysed using GC-MS. The results obtained from this study indicated that 4 out of 9 isolates were able to produce antimicrobial metabolites. The most effective isolates were R87 strain with the maximum inhibition zone of 35 mm and the least effective were R85 strain with zone of inhibition 20 mm against E. coli. The GC-Mass chromatogram showed different special peaks and the identified compounds were cyclopenthasiloxane, Azulene, 2-7 octadiene, docosane, cyclohexasiloxane, Bis (3, 5, 5 trimethyhexyl, Tetraedacane-2 methyl, Cycloheptasiloxane and Heptadecanol. In general, isolation of native bacteria from environmental samples with antimicrobial property is an interesting aspect which could help us to ignore antibiotic resistant bacteria from our environment.

Cites

References

Cite

APA: Copy

AGHAEI, S., BAHADOR, NIMA, & BASERISALEHI, MAJID. (2015). ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUND PRODUCING RHODOCOCCUS SPP. ISOLATED FROM SOIL SAMPLES. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 5(1), 463-468. SID. https://sid.ir/paper/328119/en

Vancouver: Copy

AGHAEI S., BAHADOR NIMA, BASERISALEHI MAJID. ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUND PRODUCING RHODOCOCCUS SPP. ISOLATED FROM SOIL SAMPLES. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2015;5(1):463-468. Available from: https://sid.ir/paper/328119/en

IEEE: Copy

S. AGHAEI, NIMA BAHADOR, and MAJID BASERISALEHI, “ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUND PRODUCING RHODOCOCCUS SPP. ISOLATED FROM SOIL SAMPLES,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 5, no. 1, pp. 463–468, 2015, [Online]. Available: https://sid.ir/paper/328119/en

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