IDENTIFICATION AND ISOLATION CELL WALL PROTEINS OF CANDIDA ALBICANS, CANDIDA ‎GLABRATA AND HOSPITAL SPECIMEN COMPARISON OF THEIR ELECTROPHORESIS ‎PATTERNS

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KHALILI A.; YADGARI M.H.; RIAZIPOUR M.; ROUDBAR MOHAMMADI SH.; SHOKOUH AMIRI M.R.; SHAHIN JAFARI A.

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Journal: KOWSAR MEDICAL JOURNAL Year:2007 | Volume:12 | Issue:2 Page(s): 117-125

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Title

IDENTIFICATION AND ISOLATION CELL WALL PROTEINS OF CANDIDA ALBICANS, CANDIDA ‎GLABRATA AND HOSPITAL SPECIMEN COMPARISON OF THEIR ELECTROPHORESIS ‎PATTERNS

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Keywords

CANDIDA ALBICANSQ3

CELL WALLQ3

SDS-PAGEQ3

CANDIDA GLABRATAQ3

Abstract

Objective: Since CANDIDA ALBICANS is the main cause of infection within candida factors, and ‎regarding that CANDIDA GLABRATA replacement as a producing factor is the second and third within ‎Candida factors, the aim of this research was to evaluate the protein pattern of CELL WALL of ‎CANDIDA ALBICANS and CANDIDA GLABRATA. One of the origins which can affect in causing disease is ‎organism protein especially CELL WALL protein. The objective of this research is firstly to acquire ‎Protein pattern of CELL WALL in approved factors of CANDIDA ALBICANS and CANDIDA GLABRATA in a ‎seprate manner, and secondly to obtain protein pattern of CELL WALL of CANDIDA ALBICANS gathering ‎from clinical resources. Resulting information can present of similarities and differences ‎between obtained patterns and standard patterns. The results of total protein patterns of CELL WALL ‎can be used in comparing them with patterns of cytoplasmic proteins and the results can be used ‎at In vitro and molecular studies.‎ Materials and Methods: CELL WALL proteins were separated after lysis and collected by the 2% ‎SDS (Sodium Dodecyl Sulfate) method. SDS-PAGE (Sodium Dodecyl Sulfate-Poly ‎Acrylamide Gel electrophoresis‏(‏‎ and Dice coefficient were used to evaluate the electrophoretic ‎pattern and percent homology respectively. CELL WALL proteins were obtained by the Glass Bead ‎Friction method. Conclusion: The highest level of homology in CELL WALL proteins was between nail and mouth, ‎toes and arms, sample standard C.albicans and inguinal area with 95% homology and the lowest ‎level of homology was between toes and nail with 55% homology.‎ The highest similar molecular weight in CELL WALL proteins of all isolates (35 and 64Kd) in 12 ‎specimen, (24 Kd) in 10 specimen and (50 and 26 Kd) in 9 specimen and the lowest similar ‎molecular weight in CELL WALL proteins (29, 42, 60 Kd) was observed in 8 specimen and (29 Kd) in ‎one specimen of C. albicans. Results: Results obtained from the total protein patterns of CELL WALLs can be used for comparing ‎with patterns cytoplasmic proteins. These results also can be useful for studying in vitro ‎conditions and molecular studies.

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APA: Copy

KHALILI, A., YADGARI, M.H., RIAZIPOUR, M., ROUDBAR MOHAMMADI, SH., SHOKOUH AMIRI, M.R., & SHAHIN JAFARI, A.. (2007). IDENTIFICATION AND ISOLATION CELL WALL PROTEINS OF CANDIDA ALBICANS, CANDIDA ‎GLABRATA AND HOSPITAL SPECIMEN COMPARISON OF THEIR ELECTROPHORESIS ‎PATTERNS. KOWSAR MEDICAL JOURNAL, 12(2), 117-125. SID. https://sid.ir/paper/32820/en

Vancouver: Copy

KHALILI A., YADGARI M.H., RIAZIPOUR M., ROUDBAR MOHAMMADI SH., SHOKOUH AMIRI M.R., SHAHIN JAFARI A.. IDENTIFICATION AND ISOLATION CELL WALL PROTEINS OF CANDIDA ALBICANS, CANDIDA ‎GLABRATA AND HOSPITAL SPECIMEN COMPARISON OF THEIR ELECTROPHORESIS ‎PATTERNS. KOWSAR MEDICAL JOURNAL[Internet]. 2007;12(2):117-125. Available from: https://sid.ir/paper/32820/en

IEEE: Copy

A. KHALILI, M.H. YADGARI, M. RIAZIPOUR, SH. ROUDBAR MOHAMMADI, M.R. SHOKOUH AMIRI, and A. SHAHIN JAFARI, “IDENTIFICATION AND ISOLATION CELL WALL PROTEINS OF CANDIDA ALBICANS, CANDIDA ‎GLABRATA AND HOSPITAL SPECIMEN COMPARISON OF THEIR ELECTROPHORESIS ‎PATTERNS,” KOWSAR MEDICAL JOURNAL, vol. 12, no. 2, pp. 117–125, 2007, [Online]. Available: https://sid.ir/paper/32820/en

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