TRANSIENT EXPRESSION OF COAT PROTEIN OF FOOT AND MOUTH DISEASE VIRUS (FMDV) IN ALFALFA (MEDICAGO SATIVA) BY AGROINFILTRATION

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HABIBI PIRKOOHI MAZIAR; MALEKZADEH SHAFAROUDI SAEID; MARASHI HASAN; ZIBAEE SAEID; MOHKAMI AFSANEH; NEJATIZADEH SABA

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Journal Paper

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Journal: JOURNAL OF CELL AND MOLECULAR RESEARCH Year:2016 | Volume:8 | Issue:2 Page(s): 83-89

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Title

TRANSIENT EXPRESSION OF COAT PROTEIN OF FOOT AND MOUTH DISEASE VIRUS (FMDV) IN ALFALFA (MEDICAGO SATIVA) BY AGROINFILTRATION

Author(s)

Keywords

AGROINFILTRATION

FMDV

RECOMBINANT VACCINE

ALFALFA

VP1

Abstract

An Agrobacterium -mediated transient gene expression assay was carried out in ALFALFA (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via AGROINFILTRATION procedure. The presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. Moreover, gene expression in the transformed leaves was quantified by ELISA method. The results obtained in this investigation indicated high level of gene expression in ALFALFA leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.

Cites

TRANSIENT EXPRESSION OF HA1 ANTIGEN OF H5N1 INFLUENZA VIRUS IN TOBACCO (NICOTIANA TABACUM L.) VIA AGRO-INFILTRATION

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APA: Copy

HABIBI PIRKOOHI, MAZIAR, MALEKZADEH SHAFAROUDI, SAEID, MARASHI, HASAN, ZIBAEE, SAEID, MOHKAMI, AFSANEH, & NEJATIZADEH, SABA. (2016). TRANSIENT EXPRESSION OF COAT PROTEIN OF FOOT AND MOUTH DISEASE VIRUS (FMDV) IN ALFALFA (MEDICAGO SATIVA) BY AGROINFILTRATION. JOURNAL OF CELL AND MOLECULAR RESEARCH, 8(2), 83-89. SID. https://sid.ir/paper/329281/en

Vancouver: Copy

HABIBI PIRKOOHI MAZIAR, MALEKZADEH SHAFAROUDI SAEID, MARASHI HASAN, ZIBAEE SAEID, MOHKAMI AFSANEH, NEJATIZADEH SABA. TRANSIENT EXPRESSION OF COAT PROTEIN OF FOOT AND MOUTH DISEASE VIRUS (FMDV) IN ALFALFA (MEDICAGO SATIVA) BY AGROINFILTRATION. JOURNAL OF CELL AND MOLECULAR RESEARCH[Internet]. 2016;8(2):83-89. Available from: https://sid.ir/paper/329281/en

IEEE: Copy

MAZIAR HABIBI PIRKOOHI, SAEID MALEKZADEH SHAFAROUDI, HASAN MARASHI, SAEID ZIBAEE, AFSANEH MOHKAMI, and SABA NEJATIZADEH, “TRANSIENT EXPRESSION OF COAT PROTEIN OF FOOT AND MOUTH DISEASE VIRUS (FMDV) IN ALFALFA (MEDICAGO SATIVA) BY AGROINFILTRATION,” JOURNAL OF CELL AND MOLECULAR RESEARCH, vol. 8, no. 2, pp. 83–89, 2016, [Online]. Available: https://sid.ir/paper/329281/en

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