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Information Journal Paper

Title

A Novel Signal Peptide Derived from Bacillus Licheniformis α-Amylase Efficiently Targets Recombinant Human Activin A to the Periplasm of Escherichia coli

Pages

  47-51

Abstract

 Human Activin A is a member of the transforming growth factor-β superfamily consists of two similar beta subunits. Activin A is expressed by different cells and displays numerous biological activities such as control of neuronal cell proliferation and differentiation, promotion of neuronal survival in the body. Therefore, recombinant production of Activin A is beneficial because it can be used to treat many neurodegenerative diseases such as Alzheimer's and Parkinson diseases. In this study E. coli as a cheap and fast-growing host was selected to produce recombinant human Activin A. As cytoplasmic expression of human Activin A with complex structure and disulfide bonds produces inclusion bodies, so periplasmic expression of it can be beneficial. Therefore, we used modified Iranian B. licheniformis α-amylase signal peptide as a new signal peptide in order to translocate the recombinant Activin A through the inner membrane. In this study human pro-Activin A cDNA and signal sequence were cloned in pET21b vector and resulting vector transformed into the two strains of E. coli BL21. SDS-PAGE and western blot techniques were used to confirm recombinant Activin A expression. Finally, our results indicated that the signal peptide used in this study was effective for secretion of Activin A into the periplasmic space of E. coli.

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    Cite

    APA: Copy

    Hajihassan, Zahra, HOSSEINI, SEYED KAZEM, & ZOMORODIPOUR, ALIREZA. (2018). A Novel Signal Peptide Derived from Bacillus Licheniformis α-Amylase Efficiently Targets Recombinant Human Activin A to the Periplasm of Escherichia coli. JOURNAL OF CELL AND MOLECULAR RESEARCH, 10(1), 47-51. SID. https://sid.ir/paper/329301/en

    Vancouver: Copy

    Hajihassan Zahra, HOSSEINI SEYED KAZEM, ZOMORODIPOUR ALIREZA. A Novel Signal Peptide Derived from Bacillus Licheniformis α-Amylase Efficiently Targets Recombinant Human Activin A to the Periplasm of Escherichia coli. JOURNAL OF CELL AND MOLECULAR RESEARCH[Internet]. 2018;10(1):47-51. Available from: https://sid.ir/paper/329301/en

    IEEE: Copy

    Zahra Hajihassan, SEYED KAZEM HOSSEINI, and ALIREZA ZOMORODIPOUR, “A Novel Signal Peptide Derived from Bacillus Licheniformis α-Amylase Efficiently Targets Recombinant Human Activin A to the Periplasm of Escherichia coli,” JOURNAL OF CELL AND MOLECULAR RESEARCH, vol. 10, no. 1, pp. 47–51, 2018, [Online]. Available: https://sid.ir/paper/329301/en

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