Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

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Erami Elham; Erami Elham; Sadeq zadeh Fereydoun

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Journal Paper

Paper Information

Journal: JOURNAL OF CELL AND MOLECULAR RESEARCH Year:2019 | Volume:10 | Issue:2 Page(s): 90-96

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Title

Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

Author(s)

Erami Elham | Erami Elham | Sadeq zadeh Fereydoun | Issue Writer Certificate

Keywords

Aspergillus Fumigatus

RP-HPLC

2-DE

Prefractionation

Proteome profile

Abstract

Aspergillus Fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in Proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimized Prefractionation method. Using reverse phase-high performance liquid chromatography eight fractions were separated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile of these fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically, two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expression of some proteins in A. Fumigatus. To accurately identify these proteins, further experiments are needed including Mass spectrometry analysis.

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APA: Copy

Erami, Elham, Erami, Elham, & Sadeq zadeh, Fereydoun. (2019). Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus. JOURNAL OF CELL AND MOLECULAR RESEARCH, 10(2), 90-96. SID. https://sid.ir/paper/329311/en

Vancouver: Copy

Erami Elham, Erami Elham, Sadeq zadeh Fereydoun. Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus. JOURNAL OF CELL AND MOLECULAR RESEARCH[Internet]. 2019;10(2):90-96. Available from: https://sid.ir/paper/329311/en

IEEE: Copy

Elham Erami, Elham Erami, and Fereydoun Sadeq zadeh, “Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus,” JOURNAL OF CELL AND MOLECULAR RESEARCH, vol. 10, no. 2, pp. 90–96, 2019, [Online]. Available: https://sid.ir/paper/329311/en

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