AUTOLOGOUS TRANSPLANTATION OF FROZEN-THAWED IMMATURE TESTICULAR TISSUE AFTER PUBERTY IN MICE

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BABAKHANI R.; KAZEMI ASHTIANI S.; PARIVAR KAZEM; SEDIGHI GILANI M.A.; NASRESFAHANI MOHAMMAD HOSSEIN; BAHARVAND H.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:2 Page(s): 163-173

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Title

AUTOLOGOUS TRANSPLANTATION OF FROZEN-THAWED IMMATURE TESTICULAR TISSUE AFTER PUBERTY IN MICE

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Keywords

CRYOPRESERVATIONQ3

TESTICULAR TISSUEQ2

IMMATURE MICEQ3

CHEMOTHERAPYQ3

TRANSPLANTATIONQ3

Abstract

Objective: Optimization and evaluation of CRYOPRESERVATION of immature male mice TESTICULAR TISSUE in order to obtain mature spermatozoa post CRYOPRESERVATION and grafting after puberty. Material and Methods: Following anesthetization, one testis was removed from each mouse. The TESTICULAR TISSUE was cutted into 0.5 to 1 mm3 diameters. The pieces were cryopreserved by uncontrolled long and short term slow CRYOPRESERVATION protocols by using GLY, EG and DMSO as cryoprotectants. Vitrification was also carried out by using EG and DMSO. One month post CRYOPRESERVATION, TESTICULAR TISSUEs were thawed and some pieces were examined for cell viability and others were grafted to the muscle layer of skin. 4 to 6 weeks latter, TESTICULAR TISSUE was obtained from the graft and used for histological studies. Results: Viability of Frozen-Thawed cells was 92.9±1.2% (control group), 12.7±1.4%, 15.5±3.6% and 15.2±2.9% for GLY, EG and DMSO in uncontrolled long term slow CRYOPRESERVATION protocol and 48.8±5.3%, 63.3±6.4% and 79.4±4.7% for GLY, EG and DMSO in uncontrolled short term CRYOPRESERVATION protocol and 59.2±4% and 53.6±3.3% for EG and DMSO in vitrification protocol, respectively. 23% of grafts were successful and Spermatogenic cells in different stage and complete spermatozoa were observed in these grafts.Conclusions: Results of this study showed that CRYOPRESERVATION of TESTICULAR TISSUE by short term slow CRYOPRESERVATION protocols and using DMSO resulted in high cell survival and graft of the frozen-thawed IMMATURE MICE TESTICULAR TISSUE can lead to production of mature spermatozoa.

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APA: Copy

BABAKHANI, R., KAZEMI ASHTIANI, S., PARIVAR, KAZEM, SEDIGHI GILANI, M.A., NASRESFAHANI, MOHAMMAD HOSSEIN, & BAHARVAND, H.. (2006). AUTOLOGOUS TRANSPLANTATION OF FROZEN-THAWED IMMATURE TESTICULAR TISSUE AFTER PUBERTY IN MICE. KOWSAR MEDICAL JOURNAL, 11(2), 163-173. SID. https://sid.ir/paper/33035/en

Vancouver: Copy

BABAKHANI R., KAZEMI ASHTIANI S., PARIVAR KAZEM, SEDIGHI GILANI M.A., NASRESFAHANI MOHAMMAD HOSSEIN, BAHARVAND H.. AUTOLOGOUS TRANSPLANTATION OF FROZEN-THAWED IMMATURE TESTICULAR TISSUE AFTER PUBERTY IN MICE. KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(2):163-173. Available from: https://sid.ir/paper/33035/en

IEEE: Copy

R. BABAKHANI, S. KAZEMI ASHTIANI, KAZEM PARIVAR, M.A. SEDIGHI GILANI, MOHAMMAD HOSSEIN NASRESFAHANI, and H. BAHARVAND, “AUTOLOGOUS TRANSPLANTATION OF FROZEN-THAWED IMMATURE TESTICULAR TISSUE AFTER PUBERTY IN MICE,” KOWSAR MEDICAL JOURNAL, vol. 11, no. 2, pp. 163–173, 2006, [Online]. Available: https://sid.ir/paper/33035/en

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