مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DETERMINATION OF THE MUTAGENICITY POTENTIAL OF SUPERMINT HERBAL MEDICINE BY SINGLE CELL GEL ELECTROPHORESIS IN RAT HEPATOCYTES

Pages

  245-248

Abstract

 Purpose: The increasing use of herbal drugs and their easy availability have necessitated the use of mutagenicity test to analyze their toxicity and safety. The aim of this study was to evaluate the genotoxicity of SUPERMINT HERBAL MEDICINE in DNA breakage of rat hepatocytes in comparison with sodium dichromate by single cell gel electrophoresis technique or COMET ASSAY.Methods: Hepatocytes were prepared from male wistar rats and were counted and kept in a bioreactor for 30 minutes. Then cells were exposed to the SUPERMINT HERBAL MEDICINE at doses of 125, 250 and 500 ml/ml. Buffer 4 (incubation buffer) and sodium dichromate were used as negative and positive control for one hour respectively. Then cell suspension with low melting point agarose were put on precoated slides and covered with agarose gel. Then lysing, electrophoresis, neutralization and staining were carried out. Finally the slides were analyzed with fluorescence microscope. The parameter under this analysis was the type of migration which was determined according to Kobayashi pattern.Results: With increased dose of SUPERMINT HERBAL MEDICINE the DNA damage was slightly increased (P<0001).Conclusion: In overall compared to the positive control significant differences is observed which convinced that the crude extract of SUPERMINT in vitro did not have mutagenic effect.

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    APA: Copy

    KALANTARI, HEIBATULLAH, REZAEI, MOHSEN, MAHDAVINIA, MASOUD, KALANTAR, MOJTABA, AMANPOUR, ZIVAR, & VARNASERI, GOLNAZ. (2012). DETERMINATION OF THE MUTAGENICITY POTENTIAL OF SUPERMINT HERBAL MEDICINE BY SINGLE CELL GEL ELECTROPHORESIS IN RAT HEPATOCYTES. ADVANCED PHARMACEUTICAL BULLETIN, 2(2), 245-248. SID. https://sid.ir/paper/331669/en

    Vancouver: Copy

    KALANTARI HEIBATULLAH, REZAEI MOHSEN, MAHDAVINIA MASOUD, KALANTAR MOJTABA, AMANPOUR ZIVAR, VARNASERI GOLNAZ. DETERMINATION OF THE MUTAGENICITY POTENTIAL OF SUPERMINT HERBAL MEDICINE BY SINGLE CELL GEL ELECTROPHORESIS IN RAT HEPATOCYTES. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2012;2(2):245-248. Available from: https://sid.ir/paper/331669/en

    IEEE: Copy

    HEIBATULLAH KALANTARI, MOHSEN REZAEI, MASOUD MAHDAVINIA, MOJTABA KALANTAR, ZIVAR AMANPOUR, and GOLNAZ VARNASERI, “DETERMINATION OF THE MUTAGENICITY POTENTIAL OF SUPERMINT HERBAL MEDICINE BY SINGLE CELL GEL ELECTROPHORESIS IN RAT HEPATOCYTES,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 2, no. 2, pp. 245–248, 2012, [Online]. Available: https://sid.ir/paper/331669/en

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