INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH

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ZAMANI FATEMEH; ZARE SHAHNEH FATEMEH; AGHEBATI MALEKI LEILI; BARADARAN BEHZAD

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Journal: Advanced Pharmaceutical Bulletin Year:2013 | Volume:3 | Issue:2 Page(s): 329-332

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Title

INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH

Author(s)

ZAMANI FATEMEH | ZARE SHAHNEH FATEMEH | AGHEBATI MALEKI LEILI | BARADARAN BEHZAD | Issue Writer Certificate

Keywords

CD14

25-D3

LPS

DMSO

MONOCYTE

Abstract

Purpose: CD14, one of the main differentiation markers on the surface of myeloid lineage cells, acts as a key role in activation of LPS-induced MONOCYTEs. LPS (lipopolysaccharide) binds to LPS-binding protein in plasma and are delivered to the cell surface receptor CD14. In this study, Various stimuli [Dimethyl Sulfoxide (DMSO), active 1, 25-dihydroxyvitamin D3 [1, 25 (OH) 2D3] and LPS], either alone or in combination, have been recognized that have an effect on the level of CD14 expression in the human HL-60 and U937 promonocytic cell lines and therefore induce their terminal differentiation into MONOCYTEs or mature macrophages.Methods: U937 and HL-60 cells were cultured in RPMI 1640 supplemented with 10% FBS. For each cell line, 1×106 cells were seeded for 72 hours with DMSO, 14 days with LPS and 18 days with 1, 25-D3 in each well plate; then ELISA method was used to study their responses to the factors by means of anti-CD14.Results: ELISA assay demonstrated that U937 and HL-60 cells were induced by both [1, 25 (OH) 2D3] and DMSO to obtain characteristics of adherent cells and express CD14 protein; moreover, LPS at a low dose increased CD14 expression on surface of this cells.Conclusion: According to the our results, it is speculated that CD14 gene expression may be induced in human U937 and HL-60 cell lines by different factors including 1, 25-D3, DMSO and LPS.

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APA: Copy

ZAMANI, FATEMEH, ZARE SHAHNEH, FATEMEH, AGHEBATI MALEKI, LEILI, & BARADARAN, BEHZAD. (2013). INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH. ADVANCED PHARMACEUTICAL BULLETIN, 3(2), 329-332. SID. https://sid.ir/paper/331783/en

Vancouver: Copy

ZAMANI FATEMEH, ZARE SHAHNEH FATEMEH, AGHEBATI MALEKI LEILI, BARADARAN BEHZAD. INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2013;3(2):329-332. Available from: https://sid.ir/paper/331783/en

IEEE: Copy

FATEMEH ZAMANI, FATEMEH ZARE SHAHNEH, LEILI AGHEBATI MALEKI, and BEHZAD BARADARAN, “INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 3, no. 2, pp. 329–332, 2013, [Online]. Available: https://sid.ir/paper/331783/en

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