CONSTRUCTION OF YEAST RECOMBINANT EXPRESSION VECTOR CONTAINING HUMAN EPIDERMAL GROWTH FACTOR (HEGF)

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MOHAMMADIAN JAMAL; MANSOORI DERAKHSHAN SIMA; MOHAMMADIAN MASOOD; SHEKARI KHANIANI MAHMOUD

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Journal Paper

Paper Information

Journal: Advanced Pharmaceutical Bulletin Year:2013 | Volume:3 | Issue:2 Page(s): 473-476

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Title

CONSTRUCTION OF YEAST RECOMBINANT EXPRESSION VECTOR CONTAINING HUMAN EPIDERMAL GROWTH FACTOR (HEGF)

Author(s)

MOHAMMADIAN JAMAL | MANSOORI DERAKHSHAN SIMA | MOHAMMADIAN MASOOD | SHEKARI KHANIANI MAHMOUD | Issue Writer Certificate

Keywords

EPIDERMAL GROWTH FACTOR

PPIC9

POLYMERASE CHAIN REACTION

CLONING

EXPRESSION

SEQUENCING

Abstract

Purpose: The objective of this study was construction of recombinant hEGF-pPIC9 which may be used for EXPRESSION of recombinant hEGF in following studies.Methods: EGF cDNA was purchased from Genecopoeia Company and used for PCR amplification. Prior to ligation, the PCR product and PPIC9 vector was digested with EcoRI and XhoI and ligated in PPIC9 vector and subjected to colony PCR screening and SEQUENCING analysis.Results: PCR amplification of EGF cDNA using recombinant hEGF-pPIC9 vector as template was concluded in amplification of 197bp fragment. Construction of recombinant hEGF-pPIC9 of EGf gene was verified by PCR and SEQUENCING.Conclusion: Construction of Recombinant hEGF-pPIC9 was the primary stage for production and EXPRESSION of EFG in the future study.

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APA: Copy

MOHAMMADIAN, JAMAL, MANSOORI DERAKHSHAN, SIMA, MOHAMMADIAN, MASOOD, & SHEKARI KHANIANI, MAHMOUD. (2013). CONSTRUCTION OF YEAST RECOMBINANT EXPRESSION VECTOR CONTAINING HUMAN EPIDERMAL GROWTH FACTOR (HEGF). ADVANCED PHARMACEUTICAL BULLETIN, 3(2), 473-476. SID. https://sid.ir/paper/331785/en

Vancouver: Copy

MOHAMMADIAN JAMAL, MANSOORI DERAKHSHAN SIMA, MOHAMMADIAN MASOOD, SHEKARI KHANIANI MAHMOUD. CONSTRUCTION OF YEAST RECOMBINANT EXPRESSION VECTOR CONTAINING HUMAN EPIDERMAL GROWTH FACTOR (HEGF). ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2013;3(2):473-476. Available from: https://sid.ir/paper/331785/en

IEEE: Copy

JAMAL MOHAMMADIAN, SIMA MANSOORI DERAKHSHAN, MASOOD MOHAMMADIAN, and MAHMOUD SHEKARI KHANIANI, “CONSTRUCTION OF YEAST RECOMBINANT EXPRESSION VECTOR CONTAINING HUMAN EPIDERMAL GROWTH FACTOR (HEGF),” ADVANCED PHARMACEUTICAL BULLETIN, vol. 3, no. 2, pp. 473–476, 2013, [Online]. Available: https://sid.ir/paper/331785/en

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