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Information Journal Paper

Title

CONSTRUCTION OF PPIC9 RECOMBINANT VECTOR CONTAINING HUMAN STEM CELL FACTOR

Pages

  303-308

Abstract

 Purpose: Various cytokine regulates hematopoesis; they promote number of stages in stem cells biology such as proliferation, differentiation and endurance. Biological effects of SCF, as a hematopoietic cytokine; is triggered by binding to its ligand c-kit. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. In this study we tried to construct of pPIC9 recombinant vector containing HUMAN SCF.Methods: hSCF cDNA was amplified by PCR and both hSCF cDNA and pPIC9 as yeast EXPRESSION vector (shuttle vector) digested by EcoR I and Xho I restriction enzymes. Subsequent the digestion reaction, ligation reaction was carried out. In order to verifying of pPIC9 recombinant vector containing hSCF, PCR and sequence analysis was performed.Results: The construction of recombinant EXPRESSION vector of pPIC9 containing hSCF cDNA was confirmed by sequencing method successfully.Conclusion: rhSCF/pPIC9 vector can be transformed into the Picha pastoris yeast as a eukaryotic host in order to produce HUMAN SCF at industrial scale.

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    APA: Copy

    FARHADI, BEHROOZ, SHEKARI KHANIANI, MAHMOUD, & MANSOORI DERAKHSHAN, SIMA. (2013). CONSTRUCTION OF PPIC9 RECOMBINANT VECTOR CONTAINING HUMAN STEM CELL FACTOR. ADVANCED PHARMACEUTICAL BULLETIN, 3(2), 303-308. SID. https://sid.ir/paper/331807/en

    Vancouver: Copy

    FARHADI BEHROOZ, SHEKARI KHANIANI MAHMOUD, MANSOORI DERAKHSHAN SIMA. CONSTRUCTION OF PPIC9 RECOMBINANT VECTOR CONTAINING HUMAN STEM CELL FACTOR. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2013;3(2):303-308. Available from: https://sid.ir/paper/331807/en

    IEEE: Copy

    BEHROOZ FARHADI, MAHMOUD SHEKARI KHANIANI, and SIMA MANSOORI DERAKHSHAN, “CONSTRUCTION OF PPIC9 RECOMBINANT VECTOR CONTAINING HUMAN STEM CELL FACTOR,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 3, no. 2, pp. 303–308, 2013, [Online]. Available: https://sid.ir/paper/331807/en

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