مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

ENHANCED IN VITRO ANTI-TUMOR ACTIVITY OF 5-AZACYTIDINE BY ENTRAPMENT INTO SOLID LIPID NANOPARTICLES

Pages

  367-375

Abstract

 Purpose: In this study the effectiveness of encapsulating of 5-AZACYTIDINE into the lipid nanoparticles was investigated and in vitro effect of encapsulated 5-AZACYTIDINE studied on MCF-7 cell lines Methods: 5-AZACYTIDINE -loaded SOLID LIPID NANOPARTICLES were produced by double emulsification (w/o/w) method by using stearic acid as lipid matrix, soy lecithin and poloxamer 407 as surfactant and co-surfactant respectively. Particle size, zeta potential, surface morphology, entrapment efficiency and kinetic of drug release were studied. In vitro effect of 5-AZACYTIDINE on MCF-7 cell line studied by MTT assay, DAPI staining, Rhodamine B relative uptake, and also Real time RT-PCR was performed for studying difference effect of free and encapsulated drug on expression of RARß2 gene.Results: The formulation F5 with 55.84±0.46 % of entrapment efficiency shows zero order kinetic of drug release and selected for in vitro studies; the CYTOTOXICITY of free drug and encapsulated drug in 48 h of incubation have significant difference. DAPI staining shows morphology of apoptotic nucleus in both free and encapsulated drug, Rhodamine B labeled SLNs show time dependency and accumulation of SLNs in cytoplasm. Real time qRT-PCR doesn’t show any significant difference (p>0.05) in expression of RARß2 gene in both cells treated with free or encapsulated drug.Conclusion: The results of the present study indicated that the entrapment of 5-AZACYTIDINE into SLNs enhanced its CYTOTOXICITY performance and may pave a way for the future design of a desired dosage form for 5-AZACYTIDINE.

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    • Cite

    APA: Copy

    JAHANFAR, FARHAD, HASANI, AKBAR, SHANEBANDI, DARIUSH, RAHMATI, MOHAMMAD, & HAMISHEHKAR, HAMED. (2016). ENHANCED IN VITRO ANTI-TUMOR ACTIVITY OF 5-AZACYTIDINE BY ENTRAPMENT INTO SOLID LIPID NANOPARTICLES. ADVANCED PHARMACEUTICAL BULLETIN, 6(3), 367-375. SID. https://sid.ir/paper/331824/en

    Vancouver: Copy

    JAHANFAR FARHAD, HASANI AKBAR, SHANEBANDI DARIUSH, RAHMATI MOHAMMAD, HAMISHEHKAR HAMED. ENHANCED IN VITRO ANTI-TUMOR ACTIVITY OF 5-AZACYTIDINE BY ENTRAPMENT INTO SOLID LIPID NANOPARTICLES. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2016;6(3):367-375. Available from: https://sid.ir/paper/331824/en

    IEEE: Copy

    FARHAD JAHANFAR, AKBAR HASANI, DARIUSH SHANEBANDI, MOHAMMAD RAHMATI, and HAMED HAMISHEHKAR, “ENHANCED IN VITRO ANTI-TUMOR ACTIVITY OF 5-AZACYTIDINE BY ENTRAPMENT INTO SOLID LIPID NANOPARTICLES,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 6, no. 3, pp. 367–375, 2016, [Online]. Available: https://sid.ir/paper/331824/en

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