مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

SHORT COMMUNICATION: HUMAN BONE MARROW-DERIVED MESENCHYMAL CELL REACTIONS TO 316L STAINLESS STEEL: AN IN VITRO STUDY ON CELL VIABILITY AND INTERLEUKIN-6 EXPRESSION

Pages

  335-338

Abstract

 Purpose: HUMAN BONE MARROW-DERIVED mesenchymal cell (hBMC) reactions to 316L STAINLESS STEEL (316L-SS) have never been evaluated. The objective of this study was to assess CELL VIABILITY and INTERLEUKIN-6 expression of hBMC cultures upon treatment with a 316L-SS implant.Methods: A cytotoxicity analysis was conducted with a 3- (4, 5-dimethylthiazol 2-yl) -2, 5- diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of INTERLEUKIN-6 was measured using enzyme-linked immunosorbent assay (ELISA).Results: CELL VIABILITY measurement was performed via IC50 formula. All treatment group showed a>50 % CELL VIABILITY with a range of 56, 5 - 96, 9 % at 24 hours, 51, 8-77, 3% at 48 hours and 70, 1- 120 % at 72 hours. INTERLEUKIN-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group.Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.

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    APA: Copy

    BUDIWAN ANWAR, IWAN, SANTOSO, ASEP, SAPUTRA, EKO, ISMAIL, RIFKY, JAMARI, J., & VAN DER HEIDE, EMILE. (2017). SHORT COMMUNICATION: HUMAN BONE MARROW-DERIVED MESENCHYMAL CELL REACTIONS TO 316L STAINLESS STEEL: AN IN VITRO STUDY ON CELL VIABILITY AND INTERLEUKIN-6 EXPRESSION. ADVANCED PHARMACEUTICAL BULLETIN, 7(2), 335-338. SID. https://sid.ir/paper/331882/en

    Vancouver: Copy

    BUDIWAN ANWAR IWAN, SANTOSO ASEP, SAPUTRA EKO, ISMAIL RIFKY, JAMARI J., VAN DER HEIDE EMILE. SHORT COMMUNICATION: HUMAN BONE MARROW-DERIVED MESENCHYMAL CELL REACTIONS TO 316L STAINLESS STEEL: AN IN VITRO STUDY ON CELL VIABILITY AND INTERLEUKIN-6 EXPRESSION. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2017;7(2):335-338. Available from: https://sid.ir/paper/331882/en

    IEEE: Copy

    IWAN BUDIWAN ANWAR, ASEP SANTOSO, EKO SAPUTRA, RIFKY ISMAIL, J. JAMARI, and EMILE VAN DER HEIDE, “SHORT COMMUNICATION: HUMAN BONE MARROW-DERIVED MESENCHYMAL CELL REACTIONS TO 316L STAINLESS STEEL: AN IN VITRO STUDY ON CELL VIABILITY AND INTERLEUKIN-6 EXPRESSION,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 7, no. 2, pp. 335–338, 2017, [Online]. Available: https://sid.ir/paper/331882/en

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