MOLECULAR DETECTION OF VANA AND VANB GENES IN VANCOMYCIN-RESISTANT ENTEROCOCCUS ISOLATED BY POLYMERASE CHAIN REACTION FROM THE INTESTINES OF CHILDREN ADMITTED TO THE INTENSIVE CARE UNITS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DAGHIGHI ZAHRA; TAJBAKHSH SAEED; GOUDARZI HOSSEIN; KARIMI ABDOLLAH; NATEGHIAN ALIREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Archives of Pediatric Infectious Diseases Year:2014 | Volume:2 | Issue:4 Page(s): 1-5

Download Full-Text

View:

588

Download:

168

Cites:

Information Journal Paper

Title

MOLECULAR DETECTION OF VANA AND VANB GENES IN VANCOMYCIN-RESISTANT ENTEROCOCCUS ISOLATED BY POLYMERASE CHAIN REACTION FROM THE INTESTINES OF CHILDREN ADMITTED TO THE INTENSIVE CARE UNITS

Author(s)

Keywords

ENTEROCOCCUS

VANCOMYCIN

POLYMERASE CHAIN REACTION

VANB PROTEIN

Abstract

Background: Enterococci are considered as the third most common cause of nosocomial infections and their antimicrobial resistance has been a concerning issue.Objectives: In this study, we looked for resistance genes of vanA and vanB in VANCOMYCIN-resistant ENTEROCOCCUS (VRE) isolated from intestinal colonization of children admitted to the pediatric intensive care unit (PICU) and neonatal ICU (NICU) of Ali-Asghar Children's Hospital.Patients and Methods: In this descriptive study, 71 rectal swab samples were collected from the intestines of children admitted to the PICU and NICU of Ali-Asghar Children's Hospital. Enterococci were diagnosed in samples by appropriate microbiological tests. Antimicrobial resistance and VRE detection was performed by the Kirby-Bauer disk diffusion method on Mueller-Hinton agar based on Clinical and Laboratory Standards Institute (CLSI) criteria. vanA and vanB genes were detected by PCR.Results: ENTEROCOCCUS was detected in 64 (90.1%) rectal swab samples. The frequency rate of VRE strains was 47 (73.4%) and VANCOMYCINintermediate ENTEROCOCCUS (VIE) strains was 6 (9.4%). PCR analysis of VRE samples showed that 42 samples had vanA gene (89.3%) but vanB gene was not identified in remaining five samples. VIR was detected in 4 cases with vanA gene (66.7%). Again, we did not d vanB gene in remaining samples.Conclusions: VRE colonization was very high among studied cases. Most important mechanism for high level of resistance to VANCOMYCIN is presence of van genes, which can be potentially transmittable to other enterococci and gram-positive organisms. More molecular studies are needed to clarify the trend of VRE colonization and the role of preventive measures in this setting.

Cites

No record.

References

No record.

Cite

APA: Copy

DAGHIGHI, ZAHRA, TAJBAKHSH, SAEED, GOUDARZI, HOSSEIN, KARIMI, ABDOLLAH, & NATEGHIAN, ALIREZA. (2014). MOLECULAR DETECTION OF VANA AND VANB GENES IN VANCOMYCIN-RESISTANT ENTEROCOCCUS ISOLATED BY POLYMERASE CHAIN REACTION FROM THE INTESTINES OF CHILDREN ADMITTED TO THE INTENSIVE CARE UNITS. ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES, 2(4), 1-5. SID. https://sid.ir/paper/331982/en

Vancouver: Copy

DAGHIGHI ZAHRA, TAJBAKHSH SAEED, GOUDARZI HOSSEIN, KARIMI ABDOLLAH, NATEGHIAN ALIREZA. MOLECULAR DETECTION OF VANA AND VANB GENES IN VANCOMYCIN-RESISTANT ENTEROCOCCUS ISOLATED BY POLYMERASE CHAIN REACTION FROM THE INTESTINES OF CHILDREN ADMITTED TO THE INTENSIVE CARE UNITS. ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES[Internet]. 2014;2(4):1-5. Available from: https://sid.ir/paper/331982/en

IEEE: Copy

ZAHRA DAGHIGHI, SAEED TAJBAKHSH, HOSSEIN GOUDARZI, ABDOLLAH KARIMI, and ALIREZA NATEGHIAN, “MOLECULAR DETECTION OF VANA AND VANB GENES IN VANCOMYCIN-RESISTANT ENTEROCOCCUS ISOLATED BY POLYMERASE CHAIN REACTION FROM THE INTESTINES OF CHILDREN ADMITTED TO THE INTENSIVE CARE UNITS,” ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES, vol. 2, no. 4, pp. 1–5, 2014, [Online]. Available: https://sid.ir/paper/331982/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops