MOLECULAR ANALYSIS OF 18S RRNA GENE OF CRYPTOSPORIDIUM PARASITES FROM PATIENTS LIVING IN IRAN, MALAWI, NIGERIA AND VIETNAM

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GHAFFARI SALMAN; KALANTARI NARGES

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Journal: International Journal of Molecular and Cellular Medicine Year:2012 | Volume:1 | Issue:3 Page(s): 153-161

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Title

MOLECULAR ANALYSIS OF 18S RRNA GENE OF CRYPTOSPORIDIUM PARASITES FROM PATIENTS LIVING IN IRAN, MALAWI, NIGERIA AND VIETNAM

Author(s)

GHAFFARI SALMAN | KALANTARI NARGES | Issue Writer Certificate

Keywords

18S RRNA

CRYPTOSPORIDIUM

DIARRHEA

DEVELOPING COUNTRIES

Abstract

Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S RRNA gene in CRYPTOSPORIDIUM for exact parasite identification.DNA was extracted from 26 fecal samples from which initially CRYPTOSPORIDIUM oocysts were identified by Ziehl-Neelsen acid-fast, Auramine phenol and ELISA techniques. Nested PCR, targeting the most polymorphic region of the 18S RRNA gene and genotyping was performed by restriction endonuclease digestion of the PCR product followed by nucleotide sequencing and phylogenic analysis.Among 26 isolates analyzed, three species of CRYPTOSPORIDIUM were identified, 38.5% of the isolates were C.hominis while 53.8% of the isolates were C. parvum and 7.7% of the isolates were C. meleagridis, which the last two species have the potentially zoonotic transmission. The only 11T subtype of C. hominis was demonstrated. These strains clustered distinctly into either human or animal origin regardless of the geographical origin, age, or immunity status of the patients.In summary, this work is the first report of C. meleagridis infecting human in Iran. Moreover, it suggested that multi-locus study of CRYPTOSPORIDIUM species in DEVELOPING COUNTRIES would be necessary to determine the extent of transmission of cryptosporidiosis in the populations.

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APA: Copy

GHAFFARI, SALMAN, & KALANTARI, NARGES. (2012). MOLECULAR ANALYSIS OF 18S RRNA GENE OF CRYPTOSPORIDIUM PARASITES FROM PATIENTS LIVING IN IRAN, MALAWI, NIGERIA AND VIETNAM. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 1(3), 153-161. SID. https://sid.ir/paper/332103/en

Vancouver: Copy

GHAFFARI SALMAN, KALANTARI NARGES. MOLECULAR ANALYSIS OF 18S RRNA GENE OF CRYPTOSPORIDIUM PARASITES FROM PATIENTS LIVING IN IRAN, MALAWI, NIGERIA AND VIETNAM. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2012;1(3):153-161. Available from: https://sid.ir/paper/332103/en

IEEE: Copy

SALMAN GHAFFARI, and NARGES KALANTARI, “MOLECULAR ANALYSIS OF 18S RRNA GENE OF CRYPTOSPORIDIUM PARASITES FROM PATIENTS LIVING IN IRAN, MALAWI, NIGERIA AND VIETNAM,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 1, no. 3, pp. 153–161, 2012, [Online]. Available: https://sid.ir/paper/332103/en

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