CAPABILITY OF CARTILAGE EXTRACT TO IN VITRO DIFFERENTIATION OF RAT MESENCHYMAL STEM CELLS (MSCS) TO CHONDROCYTE LINEAGE

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TALAKOOB SETAREH; JOGHATAEI MOHAMMAD TAGHI; PARIVAR KAZEM; BANANEJ MARYAM; SANADGOL NIMA

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Journal: International Journal of Molecular and Cellular Medicine Year:2015 | Volume:4 | Issue:1 Page(s): 9-21

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Title

CAPABILITY OF CARTILAGE EXTRACT TO IN VITRO DIFFERENTIATION OF RAT MESENCHYMAL STEM CELLS (MSCS) TO CHONDROCYTE LINEAGE

Author(s)

Keywords

CHONDROCYTES

CARTILAGE EXTRACT

MESENCHYMAL STEM CELLS

OMENTUM TISSUE

Abstract

The importance of MESENCHYMAL STEM CELLS (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells is of utmost importance for stem cell researches. In this study, the ability of CARTILAGE EXTRACT to induce differentiation of rat derived OMENTUM TISSUE MSCs (rOT-MSCs) into chondrocyte cells (CCs) was investigated. After isolation of rOT-MSCs, they were co-cultured with different concentrations of hyaline CARTILAGE EXTRACT and chondrocyte differentiation was monitored. Expression of MSCs markers was analyzed via flow cytometry. Moreover, expression of octamer- binding transcription factor-4 (Oct-4), Wilm's tumor suppressor gene-1 (WT-1), aggrecan (AG), collagen type-II (CT-II) and collagen type-X (CT-X) was analyzed using RT-PCR on 16, 18 and 21 days. Furthermore, immunocytochemistry and western blot were performed for CT-II production. Finally, proteoglycans (PGs) were examined using toluidine blue and alcian blue staining. The phenotypic characterization revealed the positive expression of CD90, CD44 and negative expression of CD45 in rOT-MSCs. These cells also expressed mRNA of Oct-4 and WT-1 as markers of OMENTUM TISSUE. Differentiated rOT-MSCs in the presence of 20 mg/ ml CARTILAGE EXTRACT expressed AG, CT-II, CT-X, and PGs as specific markers of CCs. These observations suggest that CARTILAGE EXTRACT is potentially able to induce differentiation of MSCs into chondrocyte lineage and may be considered as an available source for imposing tissue healing on the damaged cartilage. More investigations are needed to prove in vivo cartilage repair via CARTILAGE EXTRACT or its effective factors.

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APA: Copy

TALAKOOB, SETAREH, JOGHATAEI, MOHAMMAD TAGHI, PARIVAR, KAZEM, BANANEJ, MARYAM, & SANADGOL, NIMA. (2015). CAPABILITY OF CARTILAGE EXTRACT TO IN VITRO DIFFERENTIATION OF RAT MESENCHYMAL STEM CELLS (MSCS) TO CHONDROCYTE LINEAGE. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 4(1), 9-21. SID. https://sid.ir/paper/332147/en

Vancouver: Copy

TALAKOOB SETAREH, JOGHATAEI MOHAMMAD TAGHI, PARIVAR KAZEM, BANANEJ MARYAM, SANADGOL NIMA. CAPABILITY OF CARTILAGE EXTRACT TO IN VITRO DIFFERENTIATION OF RAT MESENCHYMAL STEM CELLS (MSCS) TO CHONDROCYTE LINEAGE. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2015;4(1):9-21. Available from: https://sid.ir/paper/332147/en

IEEE: Copy

SETAREH TALAKOOB, MOHAMMAD TAGHI JOGHATAEI, KAZEM PARIVAR, MARYAM BANANEJ, and NIMA SANADGOL, “CAPABILITY OF CARTILAGE EXTRACT TO IN VITRO DIFFERENTIATION OF RAT MESENCHYMAL STEM CELLS (MSCS) TO CHONDROCYTE LINEAGE,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 4, no. 1, pp. 9–21, 2015, [Online]. Available: https://sid.ir/paper/332147/en

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