Amniotic Fluid that Supports the Growth of Plasma Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAKHTIARI M.; KAVIANI S.; Abroon S.; MOALLEMI M.; JALAEIKHOO H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2018 | Volume:21 | Issue:4 Page(s): 304-318

Download Full-Text

Persian Verion

View:

646

Download:

Cites:

Information Journal Paper

Title

Amniotic Fluid that Supports the Growth of Plasma Cells

Author(s)

Keywords

Amniotic fluidQ1

Multiple myelomaQ1

Plasma cellQ2

ProliferationQ1

Abstract

Background: Multiple myeloma is a type of Plasma cell carcinoma, whose development depends on the interaction between malignant Plasma cells and the microenvironment that is regulated by the receptors. Although the initial adhesion of the cells, resistance to apoptosis, and increased secretion of cytokines and growth factors contribute to the survival, growth and Proliferation of myeloma cells, maintaining lung cells in the laboratory is not easy and faces certain challenges. Since conducting research on myeloma requires optimizing and maintaining their culture conditions in vitro, it appears that Amniotic fluid can promote the culture of these cells in vitro. Materials and Methods: Myeloma cells were isolated from six patients by MACS technique. Cells were cultured in media containing RPMI with 10% FBS (active and inactive) and 10%, 25%, 50% Amniotic fluid (active and passive) for 2 weeks. Cell survival was measured every other day using trypan blue staining during these two weeks. Next, the expression of Proliferation genes (BCL-2), implantation genes (CXCR4) and cell cycle stop genes (P21, P27) were studied using qualitative PCR technique. Results: The results showed that the medium containing 25% passive Amniotic fluid and 10% inactive FBS significantly increased the Proliferation of myeloma cells, and on day zero (on the day of isolation) all of these genes were expressed. Furthermore, on the fourth day of cultivation, in all groups, BCL-2 and CXCR4 genes were expressed, while P21 and P27 genes were not. This difference could indicate the effect of Amniotic fluid on the growth and Proliferation of the myeloma cells. Conclusion: According to the results, the use of a medium containing 25% inactive Amniotic fluid plus a base medium that contains active RPMI and FBS 10% is recommended for the culture of myeloma cells.

Cites

Immunophenotypic and Immunotypic Evaluations of Human Myeloma Cell Lines KMM-1, JJN3, LP1, L363, KMS-12BM and RPMI-8226 for Cell-Line Authentication

References

No record.

Cite

APA: Copy

BAKHTIARI, M., KAVIANI, S., Abroon, S., MOALLEMI, M., & JALAEIKHOO, H.. (2018). Amniotic Fluid that Supports the Growth of Plasma Cells. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 21(4 ), 304-318. SID. https://sid.ir/paper/33478/en

Vancouver: Copy

BAKHTIARI M., KAVIANI S., Abroon S., MOALLEMI M., JALAEIKHOO H.. Amniotic Fluid that Supports the Growth of Plasma Cells. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2018;21(4 ):304-318. Available from: https://sid.ir/paper/33478/en

IEEE: Copy

M. BAKHTIARI, S. KAVIANI, S. Abroon, M. MOALLEMI, and H. JALAEIKHOO, “Amniotic Fluid that Supports the Growth of Plasma Cells,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 21, no. 4 , pp. 304–318, 2018, [Online]. Available: https://sid.ir/paper/33478/en

Related Journal Papers