Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter

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NAZARI VANANI R.; Tondro G.H.; Dehdari Vais R.; HAGHKHAH M.; HELI H.; SATTARAHMADY N.

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Journal Paper

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Journal: Journal of Biomedical Physics and Engineering Year:2020 | Volume:10 | Issue:3 Page(s): 307-318

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Title

Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter

Author(s)

Keywords

Lateral Flow

Test Strip

Tuberculosis

Nucleic Acid Hybridization

Metal Nanoparticles

Gold

Abstract

Background: Mycobacterium Tuberculosis (MTB) is a pathogen causing Tuberculosis (TB) in human, and TB can cause enormous social and economic disruptions. Lateral Flow Test Strips (LFTSs) are inexpensive, portable, disposable, rapid, and easy-to-use analytical tools. Objective: LFTSs were prepared for the detection of MTB. LFTSs were fabricated using a new specific probe for MTB H37Rv, based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA). Material and Methods: In this experimental study, to create test and control zones, streptavidin (STP) and a 150-mer dA were dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) were conjugated with poly deoxythymidine sequence and placed on the conjugate pad. The composition of immersion buffers for sample pad and conjugate pad, running solution, solutions of GNPs-S-dT150 and STP were introduced. DNA genome of MTB and Mycobacterium bovis in clinical samples was amplified with PCR, and then detected by the LFTSs. During the assay, samples were firstly hybridized in two steps and then placed on a conjugate pad in a manner that positive and negative samples provided two and one red lines, respectively, on the detection pad. Results: After PCR reaction with biotinylated primer, hybridization process with specific MTB probe-dA70-100 toke 10 min, and running process on the strip was performed within 5 min. Conclusion: We showed that LFTS can discriminate a particular bacteria strain from others. The LFTSs can be redesigned for detection of other pathogenic genomes.

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APA: Copy

NAZARI VANANI, R., Tondro, G.H., Dehdari Vais, R., HAGHKHAH, M., HELI, H., & SATTARAHMADY, N.. (2020). Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter. JOURNAL OF BIOMEDICAL PHYSICS AND ENGINEERING, 10(3), 307-318. SID. https://sid.ir/paper/334785/en

Vancouver: Copy

NAZARI VANANI R., Tondro G.H., Dehdari Vais R., HAGHKHAH M., HELI H., SATTARAHMADY N.. Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter. JOURNAL OF BIOMEDICAL PHYSICS AND ENGINEERING[Internet]. 2020;10(3):307-318. Available from: https://sid.ir/paper/334785/en

IEEE: Copy

R. NAZARI VANANI, G.H. Tondro, R. Dehdari Vais, M. HAGHKHAH, H. HELI, and N. SATTARAHMADY, “Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter,” JOURNAL OF BIOMEDICAL PHYSICS AND ENGINEERING, vol. 10, no. 3, pp. 307–318, 2020, [Online]. Available: https://sid.ir/paper/334785/en

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