IMMUNOGENICITY OF RECOMBINANT ISS PROTEIN AS POTENTIAL VACCINE AGAINST COLIBACILLOSIS IN POULTRY

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DERAKHSHANDEH ABDOLLAH

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Journal Paper

Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 38-38

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Title

IMMUNOGENICITY OF RECOMBINANT ISS PROTEIN AS POTENTIAL VACCINE AGAINST COLIBACILLOSIS IN POULTRY

Author(s)

DERAKHSHANDEH ABDOLLAH

Keywords

COLIBACILLOSIS

APEC

ISS

IMMUNOGENICITY

Abstract

Colibacillosis is a disease caused by avian pathogenic Escherichia coli (APEC) and depending on the virulence status of the strain, host status and presence and type of predisposing factors, is followed by either sudden death or localized inflammation in multiple organs. Virulence factors described so far for APEC include adhesions (type 1, curli and P fimbriae), flagella, Aerobactin system, capsule, outer membrane protein, temperature sensitive haemagglutination, serum resistance and colicin production but the exact mechanism of pathogenesis is not yet fully elucidated. One of the major virulence factors, which cause resistance of APEC to serum complement, is ISS gene located on ColV plasmid. The objectives of this study were to clone, sequence, expression and immunogenicity of ISS protein. The ISS gene cloned and sequenced into pTZ57R/T and pGEX-3X vectors, after that protein expression were analyzed by SDS-PAGE and western blot. Finally ISS protein sequence was confirmed by Ms-Ms. Sequence analysis showed that the ISS gene from E. coli (O78: K80) isolated from Iranian poultry does not differ from previous reported genes submitted in GenBank database. GST: ISS fusion protein was purified by Glutathione sepharose column. To investigate immunity, purified ISS injected to animal model and checked by Dot-blot assay. The results showed that ISS protein could be used for detection and control of colibacillosis in poultry.

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APA: Copy

DERAKHSHANDEH, ABDOLLAH. (2014). IMMUNOGENICITY OF RECOMBINANT ISS PROTEIN AS POTENTIAL VACCINE AGAINST COLIBACILLOSIS IN POULTRY. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 38-38. SID. https://sid.ir/paper/335947/en

Vancouver: Copy

DERAKHSHANDEH ABDOLLAH. IMMUNOGENICITY OF RECOMBINANT ISS PROTEIN AS POTENTIAL VACCINE AGAINST COLIBACILLOSIS IN POULTRY. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):38-38. Available from: https://sid.ir/paper/335947/en

IEEE: Copy

ABDOLLAH DERAKHSHANDEH, “IMMUNOGENICITY OF RECOMBINANT ISS PROTEIN AS POTENTIAL VACCINE AGAINST COLIBACILLOSIS IN POULTRY,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 38–38, 2014, [Online]. Available: https://sid.ir/paper/335947/en

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