DIAGNOSIS OF LEPTOSPIROSIS IN HAEMORRHAGIC FEVER IN DOMESTIC ANIMALS OF THE HELLEH RIVER AREA IN 2004 BY POLYMERASE CHAIN REACTION (PCR) MOLECULAR TECHNIQUE

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AMIRI NEZHAD ROUYA; VAHDAT K.; NABIPOUR I.; JAFARI SEYED MOJTABA; GHAJARI AMR ELAH; HAJHOSSEINI R.; HOSSEINI SEYED JAVAD; ZANDI K.; ZAFARMAND M.H.; MOTAMEDI MAHDI; SANJIDEH Z.

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Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2007 | Volume:10 | Issue:1 Page(s): 9-18

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Title

DIAGNOSIS OF LEPTOSPIROSIS IN HAEMORRHAGIC FEVER IN DOMESTIC ANIMALS OF THE HELLEH RIVER AREA IN 2004 BY POLYMERASE CHAIN REACTION (PCR) MOLECULAR TECHNIQUE

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Keywords

LEPTOSPIROSISQ3

PCRQ2

HAEMORRHAGIC FEVERQ3

DOMESTIC ANIMALSQ3

Abstract

Background: LEPTOSPIROSIS is a globally important zoonotic disease with worldwide prevalence, caused by spirochetes from the genus Leptospira. Diagnosis based on serology requires testing of acute and convalescent sera. PCR techniques have high sensitivity and specificity for early diagnosis of this disease.Methods: Sera of all (49 cases) livestock breeders of the Helleh River area and 200 DOMESTIC ANIMALS were collected after 7-10 days from the outbreak of HAEMORRHAGIC FEVER in DOMESTIC ANIMALS of the Helleh River area in 2004, following torrential rains and flooded water occurrence in this area, and were tested by PCR based on 16srRNA gene. PCR method was used for 60 sera of livestock breeders from the surroundings villages as the normal control group. The human sera were tested for anti-Leptospiral antibodies using ELISA methods for Leptospira IgM. Results: Leptospiral genomes were detected in 16 cases (32.65%) of livestock breeders and 19 cases (9.5 %) of DOMESTIC ANIMALS by PCR. But, in the normal control group, Leptospiral genomes were not detected. A number of 15 cases of livestock breeders of Helleh River area were positive for IgM-ELISA. Among the PCR positive cases (16 persons), 10 cases were negative and 6 cases were positive for IgM-ELISA. Conclusion: The HAEMORRHAGIC FEVER in DOMESTIC ANIMALS of the Helleh River area in Bushehr is recognized to be LEPTOSPIROSIS. The 16S rRNA-based PCR method can recognize pathogenic Leptospira in clinical samples and provides early decisive diagnosis possibility of active infection.

Cites

DETECTION OF PATHOGENIC LEPTOSPIRA SPP. BY POLYMERASE CHAIN REACTION REACTION (PCR) TARGETING LIGB GENE

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APA: Copy

AMIRI NEZHAD, ROUYA, VAHDAT, K., NABIPOUR, I., JAFARI, SEYED MOJTABA, GHAJARI, AMR ELAH, HAJHOSSEINI, R., HOSSEINI, SEYED JAVAD, ZANDI, K., ZAFARMAND, M.H., MOTAMEDI, MAHDI, & SANJIDEH, Z.. (2007). DIAGNOSIS OF LEPTOSPIROSIS IN HAEMORRHAGIC FEVER IN DOMESTIC ANIMALS OF THE HELLEH RIVER AREA IN 2004 BY POLYMERASE CHAIN REACTION (PCR) MOLECULAR TECHNIQUE. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 10(1), 9-18. SID. https://sid.ir/paper/33694/en

Vancouver: Copy

AMIRI NEZHAD ROUYA, VAHDAT K., NABIPOUR I., JAFARI SEYED MOJTABA, GHAJARI AMR ELAH, HAJHOSSEINI R., HOSSEINI SEYED JAVAD, ZANDI K., ZAFARMAND M.H., MOTAMEDI MAHDI, SANJIDEH Z.. DIAGNOSIS OF LEPTOSPIROSIS IN HAEMORRHAGIC FEVER IN DOMESTIC ANIMALS OF THE HELLEH RIVER AREA IN 2004 BY POLYMERASE CHAIN REACTION (PCR) MOLECULAR TECHNIQUE. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2007;10(1):9-18. Available from: https://sid.ir/paper/33694/en

IEEE: Copy

ROUYA AMIRI NEZHAD, K. VAHDAT, I. NABIPOUR, SEYED MOJTABA JAFARI, AMR ELAH GHAJARI, R. HAJHOSSEINI, SEYED JAVAD HOSSEINI, K. ZANDI, M.H. ZAFARMAND, MAHDI MOTAMEDI, and Z. SANJIDEH, “DIAGNOSIS OF LEPTOSPIROSIS IN HAEMORRHAGIC FEVER IN DOMESTIC ANIMALS OF THE HELLEH RIVER AREA IN 2004 BY POLYMERASE CHAIN REACTION (PCR) MOLECULAR TECHNIQUE,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 10, no. 1, pp. 9–18, 2007, [Online]. Available: https://sid.ir/paper/33694/en

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